藏药独一味RP-HPLC色谱指纹图谱的建立及其质量评价

Establishment of Chromatographic Fingerprint and Quality Assessment of Lamiophlomis by RP-HPLC

[目的]建立独一味药材HPLC指纹图谱，研究不同地区独一味药材的质量，为其质量控制提供有效的方法。[方法]采用RP-HPLC方法梯度洗脱，测定10批不同地区的独一味样品。[色谱备件]Kromasil C18柱（250mm×4．60mm，5μm），流动相为甲醇-0．4％的磷酸水溶液（55：45），梯度洗脱程序为0～70min，甲醇的体积分数由1％线性增加至15％；70～100min内，甲醇由15％线性增加至17％；100～130min内，甲醇由17％线性增加至20％；130～150min，甲醇由20％线性增加至100％；流速为1ml/min，检测波长为254n／n，柱温为30℃。[结果]确定了独一味药材中的7个共有峰。根据聚类分析结果，将独一味药材分为3类。[结论]该研究建立的方法重复性好，可用于不同地区独一味药材的质量评价，结合含量测定可用于独一味药材的质量的全面控制。

[Objective] To establish the chromatographic fingerprint analysis for the quality control of Lamiophlomis. [Method] RP-HPLC method was applied to establish the chromatographic fingerprint. Use Kromasil C18 column, [ The mobile phase ]：methanol and 0.4% phosphoric acid, gradient elution program： 0-70 min methanol＇s volume percent（as follow） is from 1% to 15% ,70- 100 min from 15% to 17% ,100- 130 min from 17% to 20% ,130- 150 min from 20% to 100%; flow velocity： 1ml/min, temperature： 30 ℃ ,detection wavelength：254 nm. [Results]The HPLC chromatographic fingerprint of Lamiophlomis showing 7 cbaracteristic peaks was established. The 10 batches of Lamiophlomia were classified by cluster analysis. [Conclusion]The RP-HPLC fmgerprint method is repeatable ha analysis of Lamiophlomis and can be used to evaluate the quality of Lamiophlomis with quantitative assessment.