摘要
[目的]建立独一味药材HPLC指纹图谱,研究不同地区独一味药材的质量,为其质量控制提供有效的方法。[方法]采用RP-HPLC方法梯度洗脱,测定10批不同地区的独一味样品。[色谱备件]Kromasil C18柱(250mm×4.60mm,5μm),流动相为甲醇-0.4%的磷酸水溶液(55:45),梯度洗脱程序为0~70min,甲醇的体积分数由1%线性增加至15%;70~100min内,甲醇由15%线性增加至17%;100~130min内,甲醇由17%线性增加至20%;130~150min,甲醇由20%线性增加至100%;流速为1ml/min,检测波长为254n/n,柱温为30℃。[结果]确定了独一味药材中的7个共有峰。根据聚类分析结果,将独一味药材分为3类。[结论]该研究建立的方法重复性好,可用于不同地区独一味药材的质量评价,结合含量测定可用于独一味药材的质量的全面控制。
[Objective] To establish the chromatographic fingerprint analysis for the quality control of Lamiophlomis. [Method] RP-HPLC method was applied to establish the chromatographic fingerprint. Use Kromasil C18 column, [ The mobile phase ]:methanol and 0.4% phosphoric acid, gradient elution program: 0-70 min methanol's volume percent(as follow) is from 1% to 15% ,70- 100 min from 15% to 17% ,100- 130 min from 17% to 20% ,130- 150 min from 20% to 100%; flow velocity: 1ml/min, temperature: 30 ℃ ,detection wavelength:254 nm. [Results]The HPLC chromatographic fingerprint of Lamiophlomis showing 7 cbaracteristic peaks was established. The 10 batches of Lamiophlomia were classified by cluster analysis. [Conclusion]The RP-HPLC fmgerprint method is repeatable ha analysis of Lamiophlomis and can be used to evaluate the quality of Lamiophlomis with quantitative assessment.
出处
《安徽农业科学》
CAS
北大核心
2007年第31期9900-9901,9904,共3页
Journal of Anhui Agricultural Sciences
基金
西宁市重点项目(2006-G-01)