摘要
以农杆菌GV3101侵染预培养的无菌烟草叶方块,共培养2d后,外植体通过5种表面脱菌处理后转接在培养基上进行抑菌和筛选培养。结果表明,共培养后的材料先用无菌水冲洗6~8遍,以脱去材料表面大部分农杆菌,再用2%(V/V)NaClO漂洗10min,然后无菌水冲洗5遍,捞出后放在无菌滤纸上吸干多余水分,转接在抑菌和筛选培养基Ms+BA2.0mg/L+NAA0.1mg/L+Cef500mg/L+HygB20mg/L上的抑菌效果良好。
Agrobacterium tumefaciens strain GV3101 was used to infect pre-cultured sterile tobacco leaf squares. After they were co-cultured together for 2 d, the explants were transplanted in medium for bacterial-restraining and selective culture, when they were made by 5 kinds of eliminating bacteria treatments. The results showed that the co-cultured materials was washed with aseptic water for 6 - 8 times at In,st to eliminate most agrobacterium on the surfaces of materials, and bathed with 2% (V/V) NaClO for 10 min, and then washed with aseptic water for 5 times. They were put on the aseptic filter paper after fished out to sop up the superfluous water. Transplanting them in the bacterial-restraining and selective medium of MS + BA 2.0 mg/L + NAA 0.1 mg/L + Cef 500 mg/L + HygB 20 mg/L could get better bacterial-restraining effect.
出处
《安徽农业科学》
CAS
北大核心
2007年第31期9913-9914,共2页
Journal of Anhui Agricultural Sciences
基金
国家转基因植物研究与产业化开发专项(JY03A20-02)
国家自然科学基金资助项目(30170653)
关键词
遗传转化
农杆菌
抑制
Genetic transformation
Agrobacteritan
Inhibition