摘要
本文对自德国土壤中分离的8株苏云金芽孢杆菌进行毒力测定和晶体蛋白特性的研究。生物测定结果表明,8株Bt中除S21-1、S21-13对甜菜夜蛾无毒性外,对夜蛾科的4种供试昆虫均有很高的毒效,48小时校正死亡率高达80%~100%;扫描电镜观察和SDS-PAGE晶体蛋白电泳结果可知,S11-6和S21-132个菌株只有一种菱形晶体和一条相应的135KDa主带,其余6个菌株均有菱形和方形两种晶体,以及两条相应的135KDa和65KDa主带。另对晶体的特性与其毒力的关系进行了讨论。
The toxicity of eight Bacillus thuringiensis strains isolated from German soil and the character of crystal protein was reported in this paper. Bioassay results showed that all the Bt strains possessed very high toxicity to four test insects of Noctuidae. The corrected mortality at 48h reached 80% 100%. Scanning electromicroscopic observation and the pattern of SDS PAGE protein electrophoresis indicated that S 11 6 and S 21 13 strains had only one kind of bipyramidal crystal and one 135KDa main band, the other six strains had both bipyramidal and square crystal and 135KDa and 65KDa main bands. Relation of crystal protein character to its toxicity was also discussed.
出处
《中国生物防治》
CSCD
1997年第2期82-85,共4页
Chinese Journal of Biological Control
基金
广东省自然科学基金
关键词
苏云金芽孢杆菌
晶体蛋白
毒力测定
Bacillus thuringiensis Noctuidae larvae crystal proteins toxicity