^(103)Pd粒子植入对人胃癌裸鼠皮下移植瘤的杀伤作用

The lethal effect of Interstitial brachytherapy with ^(103)Pd seeds in treatment of the human gastric tumor xenografts in nude mice

目的观察^(103)Pd粒子植入对人胃癌裸鼠皮下移植瘤的杀伤作用。方法建立人胃低分化腺癌BGC-823细胞裸鼠皮下移植瘤模型,20只裸鼠随机分为实验组和对照组,实验组植入^(103)Pd粒子,根据计算机三维治疗计划系统,周边剂量为120 Gy。对照组植入冷源。分别在术后第17、34天,测量肿瘤体积,计算抑瘤率,观察病理组织学变化。结果实验组移植瘤的生长曲线呈下降趋势,而对照组明显升高。实验组植入后17 d抑瘤率为89.24%,34 d为94.77%;病理组织学反应分级为RCRG1级,与对照组比较差异有统计学意义(P<0.05),第17天与第34天比较差异无统计学意义(P>0.05)。结论^(103)Pd粒子对人胃癌裸鼠皮下移植瘤有显著杀伤效应。

To investigate the lethal effect of interstitial implantation with ^103pd seeds in nude mice with the human gastric tumor through observing the pathological changes. Methods The animal models of the human gastric poorly differentiated gland BGC-823 cell tumor were established first through the injection of the cells into nude mice subcutaneously, followed by the implantation of the palladium-103 granules into the tumor mass. The animals were divided into 2 groups randomly （ experimental and control group, 10 nude mice in each group）. The ^103Pd seeds were implanted into the tumors according to the treatment plan defined by the computer three-dimensional treatment planning system （TPS） in the experimental group, with a prescription dose of 120 Gy and a seed activity of 2.96 × 107 Bq. In the control group, the nude mice were subjected to the implantation with cold seeds. The volume of the tumor was measured, the inhibitory rate was calculated and the histopathological changes were observed at the 17th day and the 34th day after operation. Results The growth curve of the tumor in experimental group showed the decreased tendency, while in the control group, there was an increased tendency. The inhibitory rate in the experimental group was 89.24% and 94.77% after 17 and 34 days respectively, and histopathological response was RCRG 1 grade （ P 〈 0.05 ）. There was no statistically significant differences in the inhibitory rate and histopathological response between two time points （ P 〉 0.05 ）. Conclusion 103 lad has the remarkable lethal effect on the human gastric tumor xenon＇rafts in nude mice.