凋亡抑制因子XIAP在膀胱癌组织中的表达及其临床意义

Expression of XIAP in bladder cancer and its clinical significance

目的检测抗凋亡(IAP)家族中XIAP在膀胱癌组织及癌旁正常组织的表达,探讨XIAP的表达在膀胱癌发生发展中的意义。方法采用免疫组织化学和实时荧光定量逆转录-聚合酶链反应(RT-QPCR)对50例膀胱癌患者中XIAP基因在癌组织和癌旁正常组织中的表达进行检测。结果免疫染色标本中,在空白对照、癌旁正常组织和膀胱癌组织中XIAP的阳性表达率分别为0、20%和60%。RT-QPCR显示XIAP在膀胱癌组织中的-ΔΔCT值是癌旁组织的5.7163 (4.3081～7.1245)倍,与分级、分期和有无转移没有相关性,但与是否复发有相关性。结论XIAP在癌旁正常组织中有少量表达,而在膀胱癌组织中的表达量高于癌旁组织,其表达量的多少可提示肿瘤是否复发。

To study the expression of XIAP which belongs to inhibitors of apoptosis （IAP） family in bladder cancer tissue and paracancerous tissues and the clinical significance. Methods In 50 cases of bladder cancers, immunohistochemistry and real-time quantitative reverse transcription polymerase chain reaction （RT-QPCR） were used to detect the expression of XIAP in cancerous tissues and paracancerous tissues. Results The positive rate of XIAP immunostaining was 0, 20% （10/50） and 60% （30/50） respectively in blank control, cancerous tissues and cancerous tissues, respectively. The - a ACT value of XIAP in bladder cancerous tissues was 5. 7163 （4. 3081-7. 1245） times of that in paracancerous tissues. The expression of XIAP mRNA had no correlation with tumor pathological grade, clinical stage and metastasis except recurrence. Conclusion There is weak expression of XIAP mRNA in paracancerous tissues, but the expression of XIAP mRNA in bladder cancer tissue is higher than in paracancerous tissues.