摘要
目的研究桂枝芍药知母汤对Ⅱ型胶原诱导性关节炎大鼠的治疗作用,并探讨其作用机制。方法采用牛Ⅱ型胶原诱导的关节炎模型(Collagen-induced arthritis,CIA),灌胃给药21 d后观察大鼠体重变化、足肿胀度;采用ELISA法测定大鼠滑膜和血清中的白细胞介素4(IL-4)水平,小鼠巨噬细胞NO2-释放法测定大鼠滑膜和血清中干扰素γ(IFN-γ)活性。结果桂枝芍药知母汤能改善CIA大鼠体重变化,降低原发性和继发性足肿胀度,下调CIA大鼠滑膜和血清IFN-γ、IL-4水平。结论CIA大鼠滑膜中IFN-γ、IL-4水平均升高,血清中以IFN-γ升高为主。桂枝芍药知母汤抗风湿作用,可能通过下调CIA大鼠滑膜和血清IFN-γ、IL-4水平,从而调节Th1/Th2平衡。
Objective To study the effect of Guizhishaoyaozhimu Decoction（GSZD） on rats with collagen Ⅱ-induced arthritis （CIA） and to discuss the mechanism. Methods The rat model of CIA was established and then the rats were given GSZD. After 21 days the weight and swelling of ankles were measured. The level of IL-4 in synovial membrane and serum was investigated with ELISA method. The activity of IFN-γ, in synovial membrane and serum was investigated by release of NO2^- in mouse macrophage. Results The weight and swelling of CIA rats were relieved by GSZD. The level of IL-4 and the-activity of IFN-γ in synovial membrane and serum of CIA rats were down - regulated by GSZD. Conclusion The level of IL-4 and the activity of IFN-γ, in synovial membrane of CIA rats were higher, and the activity of IFN-γ, in serum was higher. GSZD has therapeutical effect on CIA rats, and one of its mechanisms may be regulating Th1/Th2 balance by decreasing IFN-γ, and IL-4 in synovial membrane and serum.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第9期618-620,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家中医药管理局中医药科学技术研究专项资助课题(No.04-05JP01)
