期刊文献+

Effects of the Sheep Polyclonal Antibodies Against the Porcine Adipocyte Plasma Membrane Proteins on Porcine Carcass Composition and Meat Quality 被引量:2

Effects of the Sheep Polyclonal Antibodies Against the Porcine Adipocyte Plasma Membrane Proteins on Porcine Carcass Composition and Meat Quality
下载PDF
导出
摘要 To detect the effects of the polyclonal antibodies raised in sheep against porcine adipocyte plasma membranes on the porcine carcass composition and meat quality, 30 pigs assigned into 6 treatment groups were given intraperitoneal injections of sheep antipig adipocyte plasma membrane immunoglobulin (ASIg) or sheep nonimmune serum immunoglobulin (NSIg). At the end of the experiment, the pigs were slaughtered at 90 kg body weight, and carcasses and meat quality were evaluated. The results showed that when pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, 20 mg purified ASIg twice at 15 and 60 kg body weight, or 20 mg purified ASIg at 60 kg body weight, respectively, their lean meat percentage, fat meat percentage, backfat thickness, loin eye area leaf fat weight, caul fat weight, heart weight, liver weight, and kidney weight were significantly affected. However, the kidney weight, lurrg weight, dressing percentage, and spleen weight did not remarkably change. Our results indicated that pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, and 20 mg ASIg twice at 15 and 60 kg body weight, have significantly different drip loss rate, cooked meat ratio, tenderness, storage loss rate, muscle fiber diameter, moisture content, dry matter content, crude protein content, and crude fat content from the control group that received 20 mg NSIg at 15 kg body weight. However, meat pH, meat color value, meat marbling score, inosinate, and myohemoglobin were not significantly affected. Our results indicated ASIg could not significantly affect the content of most muscular amino acids and intramuscular fatty acids. To detect the effects of the polyclonal antibodies raised in sheep against porcine adipocyte plasma membranes on the porcine carcass composition and meat quality, 30 pigs assigned into 6 treatment groups were given intraperitoneal injections of sheep antipig adipocyte plasma membrane immunoglobulin (ASIg) or sheep nonimmune serum immunoglobulin (NSIg). At the end of the experiment, the pigs were slaughtered at 90 kg body weight, and carcasses and meat quality were evaluated. The results showed that when pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, 20 mg purified ASIg twice at 15 and 60 kg body weight, or 20 mg purified ASIg at 60 kg body weight, respectively, their lean meat percentage, fat meat percentage, backfat thickness, loin eye area leaf fat weight, caul fat weight, heart weight, liver weight, and kidney weight were significantly affected. However, the kidney weight, lurrg weight, dressing percentage, and spleen weight did not remarkably change. Our results indicated that pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, and 20 mg ASIg twice at 15 and 60 kg body weight, have significantly different drip loss rate, cooked meat ratio, tenderness, storage loss rate, muscle fiber diameter, moisture content, dry matter content, crude protein content, and crude fat content from the control group that received 20 mg NSIg at 15 kg body weight. However, meat pH, meat color value, meat marbling score, inosinate, and myohemoglobin were not significantly affected. Our results indicated ASIg could not significantly affect the content of most muscular amino acids and intramuscular fatty acids.
出处 《Agricultural Sciences in China》 CAS CSCD 2007年第10期1256-1261,共6页 中国农业科学(英文版)
基金 the grants from the Key Project of Natural Science Foundation of Yunnan Province, China (2000C005Z) the National NaturzA Science Foundation of China (30260079).
关键词 polyclonal antibodies PIG carcass composition meat quality polyclonal antibodies, pig, carcass composition, meat quality
  • 相关文献

参考文献10

  • 1Butterwith S C,Kestin S,Griffin H D,Flint D J.Cytotoxicantibodies to chicken adipocytes and their precursors, lackof tissue specificity[].British Poultry Science.1989
  • 2De clercq L,Mourot J,Genart C,Davidts V,Boone C,RemacleC.An anti-adipocyte monoclonal antibody is cytotoxicto porcine preadipocytes in vitro and depresses thedevelopment of pig adipose tissue[].Journal of Animal Science.1997
  • 3Cryer A,Gray B R,Woodhead J S.Study on thecharacterization of bovine adipocyte precursor cells and theirdifferentiation in vitro, using an indirect-labelled-secondantibody cellular immunoassay[].Journal of DevelopmentPhysiology.1984
  • 4Dulor J,Reyne Y,Nougues J.In vivo effects of a treatmentwith antibodies to adipocyte plasma membranes in the rabbit[].Reproduction Nutrition Development.1990
  • 5Barbut S,Zhang L,Marcone M.Effects of pale, normal,and dark chicken breast meat on microstructure, extractable proteins, and cooking of marinated fillets[].Poultry Science.2005
  • 6Gao S Z,Zhang X,Ge C R,Hao J Y,Wang L,Zhang Y J.Preparation and characterization of the specific monoclonalantibodies to porcine adipocyte plasma membrane protein[].Chinese Journal of Veterinary Science.2004
  • 7Moloney A P,Allen P,Enright W J.Passive immunisationof sheep against adipose tissue: effects on metabolism, growthand body composition[].Livestock Production Science.1998
  • 8Nassar A H,Hu C Y.Characterization of polyclonalantibodies against ovine adipocyte plasma membranes[].International Journal of Biochemistry.1992
  • 9SAS Institute Inc.The Analyst Application[]..2003
  • 10Shen Q W,Means W J,Underwood K R,Thompson S A,Zhu MJ,McCormick R J,Ford S P,Ellis M,Du M.Earlypost-mortem AMP-activated protein kinase (AMPK)activation leads to phosphofructokinase-2 and -1 (PFK-2and PFK-1) phosphorylation and the development of pale,soft, and exudative (PSE) conditions in porcine longissimusmuscle[].Journal of Agriculture and Food Chemistry.2006

同被引文献22

  • 1段纲,张曦,戴志明,葛长荣,高士争.抗脂肪细胞特异膜蛋白单克隆抗体对猪内分泌代谢的影响[J].云南农业大学学报,2005,20(4):552-557. 被引量:2
  • 2VAN VLIET-OSTAPTCHOUK J V,NUOTIO M L,SLAGTER S N,et al.The pr eval ence of met abol i c syndr ome and et abol i cal l y heal t hy obesi t y i n Eur ope:a col l abor at i ve anal ysi s of t en l ar ge cohor t st udi es[J].BMCEndocr Di sor d,2014,14:19.
  • 3KOPELMAN PG.Obesity as a medical problem[J].Nature,2000,404(6778):635-643.
  • 4FLINT D J.Immunodulatory ap proaches for regulation of gr owt h and body composi t i on[J].Ani mal Pr oduct i on,1994,58(3):301-312.
  • 5FUTTER C E,PANTON D,KESTIN S,et al.Mechanism of action of cyt ot oxi c ant i bodi es t o adi pocyt es on adi pose t i ssue,l i ver and f ood i nt ake i n t he r at[J].I nt J Obes Rel at Met ab Di sor d,1992,16(8):615-622.
  • 6NASSAR A H,HU C Y.Growth and carcass characteristics of l ambs passi vel y i mmuni zed wi t h ant i bodi es devel oped agai nst ovi ne adi pocyt e pl asma membr anes[J].J Ani m Sci,1991,69(2):578-586.
  • 7MOLONEY A P,ALLEN P.Growth and weights of abdominal and carcass fat in sheep immunized against adipose cell membranes[J].Pro Nutr Soc,1989,48:14-23.
  • 8MOLONEY A P,ALLEN P,ENRIGHT W J.Passive immunisation of sheep against adipocyte tissue,effects on metabolism,growth and body composition[J].Livestock Production Science,1998,56(3):233-244.
  • 9HU C Y,SURYAWAN A,KILLEFER J,et al.Effect of antiserum to rat adipocytes on growth and body composition of the rat[J].Comp Biochem and Physiol Part A Physiol,1992,101(4):669-673.
  • 10NASSAR A H,HU C Y.Characterization of polyclonal antibodies against ovine adipocyte plasma membranes[J].Int J Biochem,1992,24(4):599-604.

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部