显微注射Ca^(2+)激活小鼠MⅡ期卵母细胞

ACTIVATION OF MOUSE OOCYTES (M Ⅱ) BY INTRACELLULAR MICROINJECTION OF CALCIUM

胞质内显微注入Ｃａ２＋可以激活小鼠ＭⅡ期卵母细胞，且随着卵龄的增加，激活率明显升高。卵龄小于１６ｈ（ｈＣＧ注射后）注射Ｃａ２＋引起的激活率很低，卵龄超过１８ｈ，激活率达到５０％以上。卵龄小于１６ｈ的卵母细胞，卵内注入超纯水未能激活卵母细胞，而在１８～１９ｈ卵龄组，注射水的对照组激活率为３５％，但显著或极显著地低于注射Ｃａ２＋的试验组。激活前注射ＥＤＴＡ卵母细胞的激活率约为３０％，激活后注射ＥＤＴＡ卵母细胞的激活率高达５０％以上，二种处理激活率出现显著差异。胞质内注入肝素，无论有无外源Ｃａ２＋。

Mouse eggs can be activated by intracellular microinjection of calcium and the levels of parthenogenetic activation induced in oocytes 12-13h,15-16h,18-19h after hCG treatment by 0.2mol/L Ca 2+ injection,and by 0.02mol/L Ca 2+ injection were 9.4%,25%,52.2%;4.8%,15.2%,62% respectively.Intracellular injection of water failed to activate oocytes removed from mice 12-16h after hCG,and 35% of water injection of oocytes removed from mice 18-19h after hCG was activated .Oocytes which were injected EDTA after ethanol treatment produced a higher rate of activation than those injected EDTA before ethanol treatment.Intracellular injection of heparin did not influence the ethanol activation rates of oocytes.