利用BIOLOG鉴定系统快速鉴定菜豆萎蔫病菌的研究

USING THE BIOLOG IDENTIFICATION SYSTEM FOR RAPID IDENTIFICATION OF BEAN WILT BACTERIUM ( CURTOBACTERIUM FLACCUM FACIENS PV. FLACCUM FACIENS )

本研究利用美国Ｂｉｏｌｏｇ公司生产的ＭｉｃｒｏＳｔａｔｉｏｎＴＭＶ３．５系统对我国一类危险性病害菜豆萎蔫病菌及其相关菌进行了快速鉴定研究。研究结果表明，来自不同国家和寄主的２４株菜豆萎蔫病菌及其相关致病变种，２３株准确鉴定至种水平，其中１３株鉴定至致病变种水平，种水平的鉴定准确率为９５．８％，另外１株至属水平。同时２株苜蓿萎蔫病菌和２株番茄溃疡病菌均鉴定至致病变种水平。经聚类分析研究，结果支持了对格兰氏阳性植病细菌在属、种水平的分类。本研究是首次使用该系统对格兰氏阳性植病细菌进行鉴定研究，试验证明Ｂｉｏｌｏｇ鉴定系统用于快速鉴定菜豆萎蔫病菌是一个很有用的工具，且由于其标准化程度高、快速准确。

The Biolog identification system (Biolog Inc. USA, BIS) with MicroStation TM V3.5 software were used for the rapid identification of bean wilt bacterium ( C. f . pv. flaccumfaciens, Cff) and related plant coryneform bacteria. The results showed that 23 of 24 isolates of Cff and related pathovars (pv.) were correctly identified to species level, one to genus level; and 13 out of 24 isolates to pv. level with an accuracy of 95.8% being identified to species level. Two isolates of Clavibacter michiganensis subsp. michiganensis and two isolates of C.m. subsp. insidiosus were also correctly identified to pv.level. Cluster analysis using MLClust program showed that Cff and related coryneform bacteria isolates seperated into two divisions and supported the classification of Clavibacter and Curtobacterium at both species and genus level.We concluded that the BIS is a useful tool for the rapid identification of Cff and meets the requirements of our port plant quarantine because of its rapid, accurate and high standards.