溃疡病菌对杨树几丁酶、β-N-乙酰氨基葡萄糖苷酶的诱导作用

CHITINASE AND β N ACETYL D GLUCOSAMINIDASE INDUCED BY DOTHIORELLA GREGARIA IN POPLARS

杨树溃疡病菌Ｄｏｔｈｉｏｒｅｌａｇｒｅｇａｒｉａ及其菌丝体提取物都能诱导杨树几丁酶、β－Ｎ－乙酰氨基葡萄糖苷酶的积累，但在诱导的速度和强度上，抗病品种和感病品种有明显的差异。抗病品种毛白杨这两种酶不仅积累的速度快，而且幅度也远大于感病品种北京杨。在Ｄ．ｇｒｅｇａｒｉａ感染实验中，毛白杨几丁酶和β－Ｎ－乙酰氨基葡萄糖苷酶活性升高的幅度分别是北京杨的４．７倍和３倍；在菌丝体提取物的诱导实验中，毛白杨这两种酶升高的幅度分别是北京杨的４．５倍和２．７倍。因此可以认为几丁酶和β－Ｎ－乙酰氨基葡萄糖苷酶与杨树对溃疡病的抗性反应有关，是其防卫系统的要素之一。

The level of chitinase and β N acetyl D glucosaminidase in poplar callus were increased by infecting with Dothiorella gregaria and treating with the mycelium extract elicitor But increase in activities of the two enzymes in resistant variety were faster and higher than those in susceptible varieties. After infection with D. gregaria , the activities of chitinase and β N acetyl D glucosaminidase in resistant variety were 4.7 times and 3 times as high as those in the susceptible ones respectively, and treating with the mycelial extract elicitor the two enzyme activities were 4.5 times and 2.7 times higher in susceptible ones respectively. The basic level (in control tests) of the two enzymes in the two poplar varieties were close. The results suggested that chitinase and β N acetyl D glucosaminidase were related with the resistant reaction of poplars to poplar canker, and were one of the factors in defence system of poplars against pathomycete.