痰液中survivin、p53蛋白检测对肺癌的诊断价值

Detection of survivin and p53 protein in sputum sample and its diagnostic

目的 探讨检测痰液中survivin、p53蛋白在肺癌诊断中的应用价值。方法 采用改良Saccomanno法收集40例肺癌患者、11例肺良性病变者和9名健康者痰液，用流式细胞技术检测痰液中survivin、p53蛋白的表达水平。结果 ①肺癌患者组痰液survivin、p53蛋白的表达量显著高于肺良性病变组和健康对照组（P〈0．05）；肺良性病变组和健康对照组痰液间无显著性差异（P〉0．05）；②痰液中p53蛋白的表达与吸烟指数有关。③痰液中survivin的表达与p53相关（r=0．575，P〈0．01）。结论 ①survivin、p53蛋白表达增高在肺癌的发生中起着重要作用。②痰或诱导痰中survivin、p53蛋白检测有可能为肺癌的诊断提供一个新的无创、安全的辅助方法。

Objective To detect the expression of survivin and p53 protein in sputum in patients with lung cancer and benign pulmonary diseases, and evaluate its value in the clinical diagnosis of lung cancer. Methods The levels of survivin and p53 protein were detected in 40 patients with lung cancer,15 patients with pulmonary benign disease and 9 healthy volunteers by flow cytometry （FCM）. The relationships between the levels of the two markers in lung cancer,pulmonary benign disease and healthy volunteers were compared. Results ① Mean levels of survivin, p53 in sputum were significantly elevated in lung cancer samples compared with those of benign diseases and volunteers （ P 〈0.05） ,but the difference of pulmonary benign diseases and healthy volunteers had no statistical significance （ P 〉0.05）. ②There was relationship between p53 protein expression in sputum and smoking index. ③The expression of survivin correlated with p53 in sputum（ r =0. 575, P 〈0.01）. Conclusions ①Survivin and p53 may play a role in the pathway of carcinogegesis. ② Detecting Survivin and p53 protein in sputum might be used as one of the new methods for diagnosis of lung cancer.