止痢颗粒抗菌作用及急性毒性实验的研究

Antibacterial Action and Acute Toxicity Tests of Zhili Granule

目的:研究止痢颗粒对大肠杆菌和痢疾杆菌感染小鼠的抗菌作用及对小鼠的急性毒性实验。方法:将小鼠随机分为阴性对照、阳性对照、止痢颗粒(2.5g.kg-1、5.0g.kg-1、10.0g.kg-1)5组,各组小鼠均腹腔注射(ip)一定量菌液,半小时后灌胃给药,每日1次,连续7天,记录各组小鼠死亡数。结果:止痢颗粒在5.8g.kg-1剂量时对大肠杆菌感染小鼠有明显的保护作用;在10.0g.kg-1剂量时对痢疾杆菌感染小鼠有明显的保护作用。急性毒性实验表明小鼠口服最大给药量相当于临床用量(15g/袋)104倍,口服安全。结论:止痢颗粒具有明显抗菌作用,口服安全无毒。

OBJECTIVE ： To study antibacterial effects of Zhili granule on mice infected with E. coli or dysentery bacilli and its acute toxicity on mice. METHODS： Mice were randomized to normal control group, positive control group and zhili granule groups （2.5g·kg^-1, 5.0g·kg^-1 and 10.0g·kg^-1 ）. All mice were injected intraperitoneally with quantitative bacterium solutions 30 minutes prior to being administered intragastrically with corresponding medicines once daily for 7 consecutive days. The number of dead mice in each group was recorded. RESULTS： At a dose of 5.8g·kg^-1 zhili granule had good anti- infective effect on mice infected by E. coli and at a dose of 10.0g·kg^-1 it had good anti- infective effect on mice infected by dysentery bacterium. Acute toxicity text showed that maximal tolerance of experimental mice to zhili granule was about 104 times as the recommendede dose （15g/bag） in clinic, and oral administration of zhili granule proved to be safe. CONCLUSION： Zhili granule had obvious antibacterial action and it was safe to use orally.