大鼠微粒皮移植创面中角蛋白19及整合素β1的异位表达

Ectopic expression of keratin 19 and integrin β1 during wound healing process after microskin grafting in rats

目的了解大鼠微粒皮移植后创面角蛋白19、整合素β1表达特征的变化,初步探讨微粒皮移植创面的愈合机制。方法取20只大鼠制成全层皮肤缺损创面模型,分为自体皮组:创面移植占缺损皮肤表皮质量10%的自体微粒皮;混合皮组:创面混合移植自、异体微粒皮,其用量分别为缺损皮肤表皮质量的10%、40%。比较移植后2、3、4周2组大鼠创面的愈合率、收缩率,观察2、4周时角蛋白19、整合素β1的表达与分布特征。结果移植后2、3周,混合皮组大鼠创面愈合率分别为(85±5)%、(84±8)%,明显高于自体皮组的(53±10)%、(65±9)%(P<0.01)。2组创面收缩率在移植后各时相点差异无统计学意义(P>0.05)。移植后2、4周,2组大鼠创面新生表皮的颗粒层和棘层均可见角蛋白19、整合素β1阳性细胞;此期间未见角蛋白19在基底层有所表达。移植后2周,2组创面基底层未见整合素β1阳性细胞;4周时,部分创面标本中有整合素β1阳性细胞在基底层间断出现。结论自、异体微粒皮混合移植有助于创面愈合。自体微粒皮和自、异体微粒皮混合移植创面中,均存在角蛋白19和整合素β1阳性细胞的异位表达。

Objective To observe the characteristics of keratin 19 and integrin β1 expressions in the wound after microskin grafting , and to investigate the healing mechanism. Methods Full layer skin defects were created in twenty Spraque-Dawley rats and they were divided into two groups, i. e, A group （ with grafting of autologous microskin accounting 10% in weight of epidermis loss from skin defect ） , B group （ with grafting of autologous mieroskin and allogeneie mieroskin, accounting 10% and 40% weight of epidermis loss respectively in skin defect ）. The wound healing rate and contraction rate were observed at 2,3,4 post-grafting week（PGW） , and the expression and distribution of keratin 19 and integrin β1 were observed at 2 and 4 PGW. Results The wound healing rate in the B group on 2 and 3 PGW was obviously higher than that in A group[（85±5）% vs （53±10）% , （84±8）% vs （65±9） % , P 〈0.01]. No obvious differenee in wound contraction rate between the two groups was observed on the 2, 3 and 4 PGW （ P 〉 0.05 ）. Ceils with expression of keratin 19 and integrin β1 were observed in the suprabasal layers of the epidermis in healing wound, but not in the basal membrane. Integrin β1 positive expression cells were not observed in the suprabasal layers until 4 PGW. Conclusion Mixed grafting with autogenous and allogenous mieroskin can improve wound healing. Eetopie expression of keratin 19 and integrinβ1 exists during wound healing process after mieroskin grafting.