摘要
用戊二醛将5-Fu与抗AFP IgG联接。该免疫联接物体外细胞毒试验表明对产生人AFP的Alexander细胞的细胞毒作用比单纯药物增强约2000倍,而对AFP阴性的K_(562)细胞的细胞毒作用与单纯药物和单纯抗体无明显差异。5例病理诊断为肝细胞肝癌,AFP阳性病人用5-Fu-抗AFPIgG免疫联接物治疗,结果有2例病人血清AFP含量下降。未发生变态反应,亦未见有明显副作用及对肝肾的损害。
Flurouacil was joined to anti-AFP IgG by glutaraldehyde. This immunoconjugate in vitro cytotoxic test showed cytotoxicity for human HCC cell line(Alexander line)which was AFP positive,but with out cytoto- xity for AFP negative K_(562) cell line.The cytotoxity of the immunoconjugate for Alexander cells was much higher(about 2000 times)than fluorouracil alone. Five pathologically proven HCC patients who were AFP positive were treat- ed with this immunoconjugate.Serum AFP level was found decreasing in two patients.No allergy was noted.No marked side effects,neither clinical ma- nifestations of the kidney nor liver damages had been observed.
关键词
抗AFP抗体
氟尿嘧啶
肝癌
Anti-AFP antibody
Flurouracil immunoconjugate
Hepatocellular carcinoma
