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系统性红斑狼疮患者程序性死亡配体-1表达的初步研究

Primary study on programmed death-ligand 1 expression in patients with systemic lupus erythematosus
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摘要 目的检测系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)程序性死亡配体-1(PD-L1)的表达水平,探讨其在系统性红斑狼疮发病过程中的作用。方法密度梯度离心法分离系统性红斑狼疮患者和正常人PBMC,采用半定量RT-PCR检测PBMC的PD-L1mRNA表达。结果系统性红斑狼疮患者活动期组和稳定期组的PD-L1mRNA表达与对照组比较明显增高,差异有显著性(P均<0.05);但PD-L1mRNA表达在系统性红斑狼疮活动期组与稳定期组之间比较无显著性差异(P>0.05)。结论系统性红斑狼疮患者外周血单个核细胞PD-L1mRNA表达水平增加,程序性死亡配体-1可能在系统性红斑狼疮的发病机制中有一定的作用。 Objective To investigate the expression of programmed deathligand 1 （ PD-L1 ） on peripheral blood mononuelear cells （PBMCs） in patients with systemic lupus erythematosus （SLE）. Methonds The PBMCs of SLE patients and controls were isolated by Fieoll density gradient medium and mRNA expression of PD-L1 was detected by half-quantitative RT-PCR. Results The expression level of PD-LlmRNA in active SLE group and inactive SLE group was found to be higher than that of the normal control group （ P 〈 0.05, P 〈 0.05, respectively ）. But there was no statistically significant difference between active SLE group and inactive SLE group （ P 〉 0, 05 ）. Conclusion The expression of PD-L1 mRNA on PBMCs in patients with SLE was significantly increased. It can be suggested that the PD-L1 might play some role in the pathogenesis of SLE.
作者 胡绍先 陈新国 熊金河 毛静 吴记平 何培根 雷小妹 韩芳 王晓璐
机构地区 华中科技大学同济医学院附属同济医院风湿免疫科 湖北省军区珞珈山干休所
出处 《临床内科杂志》 CAS 2007年第10期697-699,共3页 Journal of Clinical Internal Medicine
基金 湖北省自然科学基金资助(2000J053)
关键词 系统性红斑狼疮 外周血单个核细胞 程序性死亡配体-1 Systemic lupus erythematosus Peripheral blood mononuclear cell Programmed death-ligand 1
分类号 R593.241 [医药卫生—内科学]
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参考文献13

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二级参考文献16

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临床内科杂志
2007年 第10期

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