半夏泻心汤对应激性胃溃疡大鼠生长抑素的影响

Effect of Banxia Xiexin Decoction and Its Disassembled Recipes on Somatostatin in Rats with Stress Gastric Ulcer

目的探讨半夏泻心汤对应激性胃溃疡的作用机理。方法采用水浸-束缚应激造成大鼠急性胃溃疡模型,然后把动物分为正常组、模型组和全方组及各拆方组,观察半夏泻心汤及各拆方组对其治疗作用及对各组大鼠脑组织和胃组织生长抑素(SS)表达(免疫组化反应)的影响,为半夏泻心汤配伍规律提供科学实验研究的参考。结果与正常组(脑组织:0.0305±0.0024、胃组织0.0282±0.0037)比较,模型组(脑组织0.0237±0.0056、胃组织0.0171±0.0053)SS的表达明显下降。与模型组比较,甘补药组(脑组织0.0314±0.0027、胃组织0.0219±0.0059)和全方组(脑组织0.0294±0.0050、胃组织0.0288±0.0027)SS的表达增强,全方组的效果最明显。结论半夏泻心汤通过增加SS的表达发挥治疗作用,全方组配伍合理。

Objective To explore the acting mechanism of Banxia Xiexin Decoction （BXD） and its disas-sembled recipes on stress gastric ulcer, for providing references to the scientific researches on the assembling rule of BXD. Methods The rat model of acute gastric ulcer was established by water immersion--restraint stress. The experimental rats were divided into the normal group, the model group and the treated groups treated with BXD and its disassembled recipes respectively to observe the therapeutic efficacy and the changes of somatostatin （SS） expression in brain and gastric tissues. Results In the model group, the SS expression was 0.0237 ± 0.0056 in brain and 0.0171 ± 0.0053 in gastric tissue respectively, which was significantly lower than those in the normal group （0.0305 ± 0.0024 and 0.0282 ±0.0037） respectively. Compared to the model group, the two indexes in rats treated with full BXD were 0.0294 ± 0.0050 and 0.0288 ± 0.0027, treated with sweet flavor portion were 0.0314 ± 0.0027 and 0.0219 ± 0.0059, all showed increase of SS expression, and the increment was more significant in the former. Conclusion BXD can increase the expression of SS to realize its therapeutic efficacy, and the recipe was assembled rationally.