摘要
利用半定量RT-PCR和原位杂交的方法检测Gstm2基因在成年雄性和雌性小鼠生殖器官中的表达,并初步评价其在生殖过程中的作用。在雄性小鼠的睾丸、附睾、输精管和雌性小鼠的卵巢、输卵管、子宫、胎盘中,半定量RT-PCR的方法均检测到Gstm2的表达,在胎盘中表达水平较低,其余组织表达水平较高。利用原位杂交的方法在睾丸的间质细胞检测出较强的信号,在附睾中有微弱的信号,而输精管上皮细胞没有检测到信号;在输卵管上皮细胞和妊娠第3d的子宫上皮细胞中检出较强的信号。由于Gstm2在RNA水平在小鼠的生殖器官中广泛表达,因此我们推测Gstm2可能在小鼠精子发生、睾酮合成、精子的成熟和运输、卵子的发生和运输、胚胎着床等生殖过程中发挥作用,此结果为深入研究Gstm2在生殖生理中的功能打下基础。
The expression of Glutathione-S-transferase M2 （Gstm2） in the reproductive system of mouse was observed and the role of Gstm2 in reproductive process was evaluated. Semi-quantitative RT-PCR showed that Gstm2 expression level in the placenta was lower than that in other organs. In situ hybridization showed that Gstm2 expression varied in different tissues：the signals were strong in the stroma cell of the testis, the epithelium of the oviduct and the luminal epithelium of the uterus on day 3 of early pregnancy; there were weaker signals in the epididymis; and no signals were found in the epithelium of the spermaductus. Therefore, Gstm2 may have effects on the process of spermatogenesis, oogenesis, transportation of the gamete and zygote, and implantation. The result would be groundwork for researching the function of the Gstm2 in reproductive physiology.
出处
《动物学杂志》
CAS
CSCD
北大核心
2007年第5期27-32,共6页
Chinese Journal of Zoology
基金
国家自然科学基金项目(No.30500361)
黑龙江省教育厅科研项目(No.11511040)资助
