人溶菌酶工程菌株培养条件的研究

STUDIES ON THE CULTURE CONDITIONS OF ENGINEERED STRAIN OF HUMAN LYSOZYME

人溶菌酶在食品工业和医药上具有广泛的用途,最近发现它在癌症的继承性免疫治疗上也有作用。为使人溶菌酶达到工业化生产,我们已成功地人工合成厂人溶菌酶基因,并构建了人溶菌酶基因的重组质粒和工程菌株。为提高该工程菌人溶菌酶的表达水平,我们对影响该工程菌表达人溶菌酶的培养条件进行探讨。

The growth curves of engineered strain JPB -HLY were different in LB and RM media. The expression level and activity of human lysozyme were affected apparently by both biomass and induction time. But nalidix acid could not. The optimum pH of medium for lysozyme production was 7.5. This enzyme activity could reach 190u /ml. The enzyme protein was 60mg /L by employing an enriched growth medium and appropriate conditions.