摘要
近年来,微生物原生质体融合及诱变受到广泛重视,实验方法日趋完善,实践证明它是一种有效的育种方法。在微生物原生质体制备和再生过程中,各种微生物所需的最佳条件差别很大。我们研究厂地衣芽孢杆菌53-A,菌株原生质体形成和再生的各种因素及最佳条件,并用光学显微镜和电镜观察了原生质体形成、再生和原生质体的聚集现象,现将结果报道如下。
some factors for formation and regeneration of protoplasts of Bacillus licheniformis were studied, Under the optimum condition of formation and regeneration of protoplasts. Protoplasts of primitive strain Bacillus licheniformis 53-A6 were prepared. The rate of formation and regeneration of protoplasts of strain 53 -A6 had reached to 99.7 and 30.9 percent respectively. The formation and regeneration and the phenomenon of forming dusters were observed by optical and electron microscope.
出处
《微生物学报》
CAS
CSCD
北大核心
1997年第1期72-75,共4页
Acta Microbiologica Sinica
