表达黄瓜花叶病毒外壳蛋白的转基因番茄抗黄瓜花叶病毒侵染

TRANSGENIC TOMATOES EXPRESSING CUCUMBER MOSAIC VIRUS COAT PROTEIN ARE RESISTANT TO THE VIRUS

通过土壤农杆菌(Agrobacterium tumefaciens)介导将黄瓜花叶病毒外壳蛋白(CMV CP)的cDNA成功地引入番茄(Lycopersicon esculentum)植株中,并得到转基因植株。用强致病力CMV株系接种后,表达CMV外壳蛋白的转基因植株表现出对CMV侵染的抗性。转基因植株RI代的抗性基因以接近3:1比例分离。对R_1代接种CMV后,表达CMV CP的植株病症减轻,发病率、病情指数及病毒积累量明显低于对照。病症出现推迟1个多月。

cDNA of cucumber mosaic virus coat protein (CMV CP) was successfully introduced into tomato (Lycopersicon esculentum) plants by Agrobacterium tumefaciens mediated. Many transgenic plants were obtaind and these plants expressing CMV CP were highly resistants to CMV infection. cDNA of CMV CP in the genome of progeny R1 separated with 3: 1. Progeny R, of transgenic tomatoes expressing CMV CP were protected from CMV infection. Symptom development and virus accumulation were reduced or absent in the transgenic plants expressing CMV CP. The disease ratio and disease index of transgenic plants were much lower than the controls and symptom development was delayed for more than one month compared to those of the controls.