摘要
通过DAPI(4’,6-diamidino-2-phenylindole)染色的压片,对银杏(Ginkgo biloba L.)小孢子的形成过程进行了观察,发现小孢子母细胞减数分裂过程中,有的细胞在中期Ⅰ、中期Ⅱ时出现染色体排列异常和后期染色体桥等畸变。所形成的四分体中,具有一定数量的微核;个别植株的四分体中可有高达6.5％的微核;具微核的小孢子很可能败育。结合古植物方面的研究结果,银杏小孢子形成过程的这些异常现象为认识银杏的演化趋势提供了一定的资料。
During the study on microspore formation and its development in Ginkgo biloba L. the authors have noticed in DAPI stained squashed specimen, some abnormal chromosome arrangements appeared in metaphase Ⅰ and metaphase Ⅱ . There were also some chromosomal bridges in telophase Ⅰ and Ⅱ and some rnicronuclei in tetrads too. One of the five observed trees even has abnormality frequency as high as 6.5% . These micronucleus-contained cells could subsequently become sterile. Study of such diversities, when referred to that in paleobotany may provide insight into the evolution trends of this species.
基金
国家自然科学基金
关键词
银杏
小孢子
微核
进化
Ginkgo biloba, Microspore, Micronucleus
