摘要
用植物微管专一性解聚剂甲基胺草磷(APM)预处理蚕豆(Vicia faba L.)下表皮,再用诱导气孔运动的因子处理,在显微镜下观察气孔孔径的变化。结果显示,用50mg/L APM预处理开放或关闭状态气孔,虽胞质微管被解聚,但气孔孔径没有发生明显的变化,表明胞质微管与开放或关闭状态气孔的维持无关;而去掉APM后,CaCl_2可在4h内诱导气孔关闭,气孔的运动功能又可恢复。进一步的研究表明,开放气孔经APM预处理60min后,再用ABA、Ca^(2+)及暗处理均不能诱导气孔关闭,表明微管可能参与了ABA、Ca^(2+)及暗诱导的气孔关闭过程;关闭气孔经50mg/L APM预处理后,光诱导气孔开度较不经 APM处理的有明显差异,且随着APM预处理时间和浓度的变化,气孔开放程度亦不同,表明微管也参与了光诱导的气孔开放过程。
Roles of microtubules (MT) in regulating stomatal movements of Vicia faba L. were investigated. The results indicated that apertures of open or closed stomata were not significantly influenced by pretreatment with amiprophos-methyl (APM) 50 mg/L, a specific inhibitor of plant MT polymerization, for 60 min. However, the induction of stomatal movements was inhibited by pretreatment with APM. The inhibition was reversible after APM was removed for 4 h. The induction of stomatal closure by ABA, Ca2+, or darkness was inhibited by pretreatment with APM 50 mg/L for 60 min in the light, suggesting that MT might play roles in stomatal closure. The stimulation of stomatal opening by light was inhibited by pretreatment with APM in the dark, suggesting that MT might regulate stomatal opening as well.
基金
国家自然科学基金资助项目
