角蛋白K18及33、52位丝氨酸磷酸化的K18在HBV感染肝脏疾病中的表达及意义

Expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance

目的研究K18及Set33和Set52磷酸化的K18在乙型肝炎病毒感染后肝病不同进程肝组织中的表达及其意义。方法组织化学免疫荧光法检测K18及Set33和Set52磷酸化K18在正常肝组织、乙型肝炎病毒感染后肝硬化及重型肝炎肝组织中的表达及定位。结果K18、Set33和Set52在正常肝组织、乙型肝炎肝硬化及重型肝炎肝组织中都有表达，K18在三种肝组织中表达量没有明显差异，Set33和Set52磷酸化的K18在正常肝组织中的表达基本位于细胞质膜附近，在肝硬化、重型肝炎时表达量增加并弥漫进入细胞质中。结论Set33和Set52磷酸化的K18的表达随着HBV感染后肝脏损伤程度增加而增高，Set33和Set52磷酸化的K18是乙型肝炎病毒感染后肝脏疾病病情进展的标志。

Objective To investigate the expression of K18, Ser-33 and Ser-52 phosphorylated K18 in HBV infected human liver disease and its significance. Methods The expression and localization of K18 and Ser- 33, Ser-52 phosphorylated K18 in healthy liver tissue, in liver tissues of patients with post-HBV infection cirrhosis and severe chronic hepatitis were detected by histochemistry. Results K18, Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients and severe chronic hepatitis cases. The expression of K18 in the liver cells from the 3 different sources had no significant difference in levels. Ser-33 and Ser-52 phosphorylated K18 were expressed in normal liver cells, in liver tissues of cirrhosis patients chronicity HBV hepatitis and severe chronic hepatitis cases. Ser-33 and Ser-52 located around cytoplasmic menbrane, diffused into cytoplasm and expressed at a higher levels in cirrhosis and severe chronic hepatitis. Conclusion The expression levels of Ser-33 and Ser-52 phosphorylated K18 increased along with the progression of HBV infected human liver disease. The phosphroylation of K18 could be a marker of progression of HBV infected human liver disease.