卢龙县一起人杯状病毒引起急性胃肠炎暴发的流行病学调查

Epidemiological investigation of an outbreak of acute gastroenteritis caused by human calicivirus in Lulong CountyGastroenteritis; Disease outbreaks; Calicivirus, human

目的调查河北省卢龙县急性胃肠炎暴发的流行情况，分析发病原因。方法应用流行病学调查方法对卢龙县发生一起暴发性急性胃肠炎进行调查，采用ELISA和RT-PCR方法进行人杯状病毒检测，并对PCR产物克隆测序，进行遗传进化分析。结果在调查的736人中，发病134例，罹患率为18．20％，1例死亡。发病年龄范围在1-77岁，以青壮年发病较多。6月25～30日为发病高峰。6份粪便标本中，ELISA方法检出3份杯状病毒阳性，RT-PCR方法确认1例，并鉴定为诺如病毒GI/2基因型。结论证实由杯状病毒引起急性胃肠炎暴发。

Objective To survey an outbreak of acute gastroenteritis in Lulong County and analyze the cause of the diasease. Methods Epidemiological methods were applied to investigate an outbreak of acute gastroenteritis occurred in June 2000 in Lulong County. Stool specimens were collected from diarrhea patients and were tested for human calicivirus by ELISA and RT-PCR. The products of RT-PCR were cloned and sequenced, then phylogenetic analysis was carried out. Results In total, 736 farmers were surveyed, among them 134 had acute gastroenteritis, the attack rate was 18.20%, and one elderly patient died. The age of patients was from 1 to 77 years and the incidence of the disease among young people was higher with a peak in June 25 through 30. Six stool specimens were tested for caliciviruses by ELISA and 3 were positives, one of them was confirmed by RT-PCR and belonged to norevirus genotype G I/2. No other pathogens were detected. Conclusion Human calicivirus was confirmed to be the cause of the outbreak of acute gastreenteritis.