ADAM28在成骨样细胞MC3T3-E1中的表达

The expression of ADAM28 on osteoblast-like cells MC3T3-E1

目的:研究ADAM28在成骨样细胞MC3T3-E1中的表达。方法:采用细胞培养,免疫组化和免疫荧光染色方法检测ADAM28在成骨细胞中可能存在的机制。结果:ADAM28成功表达于小鼠MC3T3-E1成骨样细胞上,免疫组化和免疫荧光检测显示:ADAM28在MC3T3-E1上表达阳性,对照组与实验组间p<0.01,有显著性差异;荧光显微镜下,可见实验组显示较强的绿色荧光,阳性表达明显。结论:ADAM28可能通过参与成骨细胞的生物学活动来促进其细胞的增殖分化。

Objective：To study the expression of ADAM28 on osteoblast-like cells （MC3T3-E1） in vitro. Method： cell culture, immunohistochemistry and immunofluorescence were used to examine the possible mechanisms of ADAM28 on the osteoblast. Result：ADAM28 were successfully expressed on the mice osteoblast-like cells, MC3T3-El in vitro. Immunohistochemistry and immunofluorescence indicated that the expression of ADAM28 on the MC3T3-E1 were positive. P value between control group and experiment group less than 0.01,and these groups were significantly different;The experiment group of immunofluorescence shown that the cells have green fluorescence with organized structure under the fluorescence light microcopy. Conclusion：ADAM28 may promote proliferation and differentiation of oetsoblast by participating in bioactivity of oetsoblast.