鲍氏不动杆菌β-内酰胺酶、氨基糖苷类修饰酶、氯己定、磺胺耐药基因研究

Genotyping of β-Lactamases,Aminoglycoside Modifying Enzymes and Chlorhexidine-sulfanilamide from Acinetobacter baumannii

目的了解20株鲍氏不动杆菌(ABA)β-内酰胺酶、氨基糖苷类修饰酶编码基因、氯己定、磺胺耐药基因存在状况。方法对20株ABA进行了9种β-内酰胺酶基因、3种氨基糖苷类修饰酶基因和氯己定、磺胺耐药基因检测,以耐药基因为分子标记作检测结果的样本聚类进行菌株亲缘性分析。结果20株ABA中blaTEM阳性13株(65%)、blaADC阳性12株(60%)、blaSHV阳性1株(5%),其他β-内酰胺酶基因均阴性;20株ABA中氨基糖苷类修饰酶基因aac(3)-Ⅰ阳性12株(60%)、aac(6′)-Ⅰ阳性13株(65%)、ant(3″)-Ⅰ阳性14株(70%);14株检出qacE△1-sul1基因(70%),以耐药基因为分子标记作检测结果的样本聚类分析显示,其中9株为克隆传播。结论该20株菌β-内酰胺类、氨基糖苷类抗菌药物耐药与产β-内酰胺酶和氨基糖苷类修饰酶密切相关,并存在克隆传播。

OBJECTIVE To investigate the coding genes of β-1actamases,aminoglycoside modifying enzymes and the drug-resistant to chlorhexidine-sulfanilamlde genes on 20 Acinetobacter baumannii isolates in Xinjiang. METHODS Twenty strains of A. baumannii were isolated from hospitalized patients, and 9 kinds of β-lactamases genes, 3 kinds of aminoglycoside modifying enzymes genes and drug-resistant to chlorhexidine-fiulfanilamide genes were detected. The drug-resistant to chlorhexidine-sulfanilamide genes were labeled and cluster analysis was performed to analyze the affinity of strain. RESULTS The detection rates of β-lactamases coding genes of TEM, ADC and SHV groups were 65%, 60% and 5%, respectively. The others were not found in all 20 isolates tested. The detection rates of aminoglycoside modifying enzymes coding genes of aac（3）- Ⅰ, aac（6′）- Ⅰ and aac（3″）-Ⅰ were 60%, 65%and 70%, respectively. And the detection rates of qacEΔ1-stull genes were 70%. There were 9 strains showed clone transmission according to cluster analysis. CONCLUSIONS Drug-resistance of the 20 strains to β-lactam and aminoglycosides is connected with β-lactamases and aminoglycoside modifying enzymes, and there exists clone transmission.