大肠埃希菌β-内酰胺酶、质粒AmpC酶与Ⅰ类整合酶基因研究

AmpC Genes,β-Lactamases Genes and intⅠGenes in Escherichia coli

目的探讨大肠埃希菌β-内酰胺酶、质粒AmpC酶与Ⅰ类整合酶基因的存在状况。方法采用M-H药敏平板对40株临床分离的大肠埃希菌进行抗菌药物敏感试验,PCR方法检测8种β-内酰胺酶基因、2种质粒AmpC酶与Ⅰ类整合酶基因。结果40株大肠埃希菌呈多重耐药,β-内酰胺酶基因TEM、SHV、LEN、CTX-M-1、CTX-M-9、VEB、GES、PER阳性率分别为55.0%、7.5%、55.0%、12.5%、45.0%、5.0%、45.0%、35.0%,质粒AmpC酶基因ACT-1、DHA阳性率分别为57.5%、40.0%,Ⅰ类整合酶基因intⅠ1阳性率为47.5%。结论临床分离的大肠埃希菌多重耐药严重,β-内酰胺酶基因、质粒AmpC酶基因、Ⅰ类整合酶基因携带率高。

OBJECTIVE To investigate the plasmid AmpC genes,β-lactamases genes and int I genes in Escherichia coll. METHODS M-H test was performed to detect the antimicrobial susceptibility of 40 strains of E. coli isolated from the clinic.β-Lactamases genes, plasmid ArapC genes and int I genes were detected by PCR methods. RESULTS Forty strains of E. coli were multiple-drug-resistant. The positive rate of genes of TEM, SHV, LEN, CTX-M-1, CTX-M-9, VEB, GES and PER was 55.0%, 7.5%, 55.0%, 12.5%, 45.0%, 5.0%, 45.0% and 35.0%, respectively. The positive rate of plasmid AmpC genes of ACT-1 and DHA was 57. 5% and 40. 0%, respectively. The positive rate of int Ⅰ genes was 47. 5%. CONCLUSIONS E. coli isolated from the clinic is multiple-drug-resistant and wityh high positive rate of the plasmid AmpC genes, β-lactamases genes and int Ⅰ genes.