HBsAg阴性献血者HBV-DNA检测的临床意义

HBV-DNA Detection in HBsAg Negative Blood Donors and Its Clinical Significance

目的检测献血者乙型肝炎五项标志物和HBV-DNA,探讨HBV-DNA检测对于输血安全的重要性。方法应用酶联免疫法检测乙型肝炎五项标志物;应用PCR实时定量荧光检测技术检测HBV-DNA。结果1698例HBsAg阴性献血员样本,HBV-DNA检测总阳性率为2.71%,其中HBsAb(+)、HBcAb(+)模式组占5.71%,HBV-DNA阳性率为7.22%;HBsAb(+)、HBeAb(+)、HBcAb(+)模式组占3.60%,HBV-DNA阳性率为8.20%;HBcAb(+)组占6.60%,HBV-DNA阳性率为4.46%;HBeAb(+)、HBcAb(+)模式组占2.77%,HBV-DNA阳性率为8.51%;HBsAb(+)模式组占38.48%,HBV-DNA阳性率为1.21%;全阴模式组占46.94%,HBV-DNA阳性率为2.26%。结论对于HBsAg阴性的献血者,HBV-DNA检测对于保证输血安全有重要意义,应该探索更为经济、合理、有效的检测方法。

OBJECTIVE Through detecting hepatitis B virus markers and hepatitis B virus DNA, to probe into the significance of HBV-DNA detection in HBsAg negative blood donors for blood transfusion safety. METHODS ELISA was used for detecting hepatitis B virus markers, and real-time fluorescent quantitative PCR assay was introduced for measuring HBV-DNA. RESULTS Totally 1 698 samples from HBsAg negative blood donors were examined, the positive rate of HBV-DNA was 2. 71%. The rate of the HBsAb positive and HBcAb positive group, the HBsAb positive, HBeAb positive and HBcAb positive group, the HBcAb positive group, the HBeAb positive and HBcAh positive group, the HBsAb positive group and the all hepatitis B virus markers negative group were 5.71%, 3. 60%, 6.60%, 2.77%, 38.48% and 46.94%, respectively. The positive rate of HBV-DNA in these groups were 7.22%, 8, 20%, 4, 46%, 8.51%, 1.21% and 2.26%, respectively. CONCLUSIONS It＇s very important for blood transfusion safety to detect HBV-DNA in HBsAg negative blood donors. The more economic, reasonable and effective detecting method should be developed for blood transfusion safety.