远志内生真菌分离鉴定及活性筛选

Isolation,Identification and Bioactivity Screening of Endophytic Fungus Associated with Polygala tenuifolia Willd

从两个不同生长时期野生远志中分离内生真菌菌株88株,隶属于28个属,研究了各菌株对3种指示菌的拮抗作用。结果表明,远志不同生长时期不同部位的内生真菌数量、分布、种群存在差异,其优势属为Alternaria Nees。茎中内生真菌种类较多。88株内生真菌中有73株菌至少能拮抗1种指示菌,占总菌数的83.0%。4株抗性较强的菌株分别隶属于Trichothecium Link、Cephalosporium Corda、Alternaria Nees、Dactuliophora C.L.等。

Eighty-eight strains of endophytic fungus, belonging to 28 genera, were isolated from wild Polygala tenuifolia Willd of two different growth stage, and the inhibitory activities screening to three species of microbe were conducted research. The results showed that the quantity, population and distribution of the endophytic fungi varied in different tissue and different growth stage of P. tenuifolla. The species of endophytic fungus distributed in stem were more than in root and leaf, and the dominant genus was Alternaria Nees. Seventy-three strains, accounting for 83.0% of all the isolates, presented antimicrobial activities to the tested indicators. High antimicrobial activities were found in four strains, which seperatedly belonged to the genera as follows： Trichothecium Link, Cephalosp-orium Corda, Alternaria Nees, Trichosporiella Kamyschko. et al.