禽流感病毒NP蛋白与T4噬菌体SOC蛋白的融合表达

Fused Expression of AIV NP Gene And T4 Phage SOC Gene

利用PCR技术扩增禽流感病毒(avian influenza virus,AIV)NP基因。将NP基因克隆至T4噬菌体表达质粒pSOC的SOC基因(T4噬菌体表面非结构蛋白基因)下游,构建成T4噬菌体SOC位点表达NP的表达载体pSOC-NP。将其转化至大肠杆菌BL21(DE3),经IPTG诱导后表达的目的蛋白SOC-NP进行SDS-PAGE与Western-blot方法检测。结果表明,表达的SOC-NP蛋白相对分子质量约58kD,表达量占菌体总蛋白量的20.34%,并具有与AIV特异性抗体反应的活性。

NP gene of AtV was amplified by PCR and cloned into the expressing plasmid pSOC, where NP gene was fused to 3＇end of T4 phage small outer eapsid gene encoding SOC protein. T4 phage expression vector named pSOC-NP was constructed and used to transform E. coli BL21 （DE3）. The recombinant E. coli BL21 （ DE3 ） was induced by tPTG. The expressed fusion protein SOC-NP was detected by SDS-PAGE with expected molecular size of 58 kD and the expression product accounted for 20.34% of the total bacterial protein. The immunological test applying Western blot indicated that SOC-NP fusion protein could react to AtV specifically.