应用16S rDNA克隆文库法分析有机物料腐熟菌剂细菌组成

Bacteria Composition of Organic Matter-decomposing Inoculant Analyzed with 16S rDNA Clone Library

应用16SrDNA克隆文库法对有机物料腐熟菌剂A和B样品中的细菌组成进行分析研究。结果表明,样品A有14个OTU,主要是融合乳杆菌(Weissella confusa)、枯草芽孢杆菌(Bacillus subtilis)和短小芽孢杆菌(Bacillus pumilus),其比例分别占总克隆文库的28.6%、30.4%和23.2%;样品B有43个OTU,主要是布氏乳杆菌(Lactobacillus buchneri)、香肠乳杆菌(Lactobacillus farciminis)和耐酸乳杆菌(Lactobacillus acetotolerans),占总克隆文库的比例分别为18.03%、18.86%和13.12%;所得出的结果均与产品标注存在差异,样品A未提及细菌的种类,而样品B只标注短小芽孢杆菌。研究表明这一方法在微生物菌剂细菌组成分析及其质量检测中具有良好的应用前景。

Using 16S rDNA clone library method, the bacteria composition of the Organic Matter-decomposing Inoeulants A and B were investigated. The results indicated that： Sample A was clustered into 14 taxonomic operational units （OTUs）, the dominant communities are Weissella confuse, Bacillus subtilis and Bacillus pumilus, which accounted for 28.6%, 30.4% and 23.2% ; Sample B was clustered into 43 OTUs, the dominant communities are Lactobacillus bachneri, Lactobacillus farciminis and Lactobacillus acetotolerans, which accounted for 18.03%, 18.86% and 13.12%, respectively. The results had much difference from the samples＇ labels：there were not bacteria in the lable of sample A and only Bacillus pumilus was the same with the lable of sample B. This study showed that 16S rDNA clone library method has nicer application perspectives in analying the bacteria of microbial inoeulant and its quality control.