鸭跖草叶点霉粗毒素的提取方法和产毒条件的研究

Study on the Extracting Method and Producing Conditions of Phyllosticta commelimecola Toxin

以石油醚、乙酸乙酯、四氯化碳三种极性不同的溶剂对鸭跖草叶点霉病原菌的菌丝和培养液进行萃取,获取粗毒素。结果表明,叶点霉菌产生的毒素既有胞外毒素,又有胞内毒素,且用极性中等的乙酸乙酯萃取效果最好。大豆培养基和PSK分别是适合菌株生长和毒素分泌的固体培养基和液体培养基。确定有利于叶点霉产毒的温度为32℃、培养时间为14d、培养方式为150r/min振荡培养。

The crude toxin was extracted from hypha and culture solution of Phyllosticta commelimecola through three different polarity solvent： benzinum, puncificatum ethyl acetate and chloroform. The result indicated that the toxin secreted by Phyllosticta commelimecola not only was in hypha but also in culture solution and the extracting effect of ethyl acetate was the best. The soybean median and PSK media can be respectively used as solid and liquid culture media to produce toxin and grow mycelium. The optimal cultural conditions for producing toxin were temperature 32℃, cultured period 14d, cultured ways shaking of 150r/min.