葡萄糖及胰岛素对巨噬细胞清道夫受体BⅠ表达的影响

Effects of glucose and insulin on expression of scavenger receptor class B type Ⅰ in U937 macrophages

用不同浓度葡萄糖和(或)胰岛素处理U937巨噬细胞,研究其对巨噬细胞清道夫受体(SR) BⅠ表达的影响,结果表明基础生理水平胰岛素促进巨噬细胞SR-BⅠ蛋白表达,而胰岛素浓度达到100μU/ml以上时,SR-BⅠ表达反而下降。胰岛素水平的变化对SR-BⅠmRNA表达无显著影响。葡萄糖与胰岛素水平同时升高对巨噬细胞SR-BⅠ明显的下调作用也许与代谢综合征同时存在2型糖尿病患者动脉粥样硬化发生较早,发展迅速有关。

[ Summary] U937 macrophages were cultured with various concentrations of glucose and/or insulin for 24 h. Cell membrane scavenger receptor （SR）-BⅠ protein and mRNA were detected by Western blot and RT-PCR. The results showed that basal physiological insulin levels promoted SR-BⅠprotein expression of macrophages and high concentration of insulin significantly downregulated SR-BⅠ , but the transcription of SR-BⅠ mRNA did not change. High glucose and high insulin accelerates atherosclerosis through synergetically downregulating SR-BⅠ protein expression, which may cause early onset and rapid progression of atherosclerosis in the patients with metabolic syndrome and type 2 diabetes mellitus.