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RNA干扰基因敲低雌激素受体β对雌激素诱导人成骨样细胞护骨素表达的影响 被引量：2

Effect of estrogen receptor β knocked down by RNA interference on osteoprotegerin expression in estrogeninduced human osteoblast-like cells
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摘要 RNA干扰敲低人成骨样细胞株MG63中雌激素受体β后,RT-PCR法发现不同浓度的17β-雌二醇均能上调MG63细胞中的护骨素mRNA表达水平,其中以10^(-7)mol/L的作用最强。而这种作用不能被G蛋白抑制剂suramin所抑制。提示雌激素受体β可能不涉及雌激素上调MG63细胞护骨素表达的调控作用。 [Summary] After-estrogen receptor β （ERβ） in MG63 cell was knocked down by RNA interference, expression of osteoprotegerin （OPG） induced by 17β-estradiol was assayed by RT-PCR. 17β-estradiol with various concentration obviously upregulated the expression of OPG mRNA of MG63 cell with the maximal effect at the concentration 10^-7 mol/L, which was not inhibited by suramin （G protein inhibitor）. The present result suggested that ERβ was not involved in regulation of OPG mRNA expression in MG63 cell by 17β-estradiol.
作者 武兆忠 刘敏 冯鉴强 林伟 张昊 吴长伟 王金玉
机构地区 广州医学院第二附属医院核医学科医学影像实验室 广州中山大学第一附属医院耳鼻咽喉科医院 广州中山大学中山医学院生理教研室
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2007年第5期456-458,共3页 Chinese Journal of Endocrinology and Metabolism
基金 广东省科技计划项目基金(200283110102) 广东省医学科学研究基金(A2002308) 广州市属高校科技计划项目基金(1031)
关键词 RNA干扰 护骨素 雌激素受体Β 人成骨样细胞 RNA interference Osteoprotegerin Estrogen receptor β Human osteoblast-like cells
分类号 R580 [医药卫生—内分泌]
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参考文献18

  • 1Simonet WS, Lacey DL, Dunstan CR, et al. Osteoprotegerin: a novel secreted protein involved in the regulation of bone density. Cell, 1997, 89:309-319.
  • 2Tsuda E, Goto M, Mochizuki S, et al. Isolation of a novel cytokine from human fibroblasts that specifically inhibits osteoclastogenesis. Biochem Biophys Res Commun, 1997,234:137-142.
  • 3Liu BY, Guo J, Lanske B, et al. Conditionally immortalized murine bone marrow stromal ceils mediate parathyroid hormone dependent osteoclastogenesis in vitro. Endocrinology, 1998,139 : 1952-1964.
  • 4Bord S, Ireland DC, Beavan SR, et al. The effects of estrogen on osteoprotegerin, RANKL, and estrogen receptor expression in human osteoblasts. Bone, 2003,32 : 136-141.
  • 5武兆忠,刘敏,冯鉴强.RNA干扰抑制雌激素受体α亚基对雌激素诱导人成骨样细胞护骨素表达的影响[J].中国老年学杂志,2005,25(2):179-182. 被引量：2
  • 6Yasuda H, Shima N, Nakagawa N, et al. Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci USA, 1998,95 : 3597-3602.
  • 7Lacey DL, Timms E, Tan HL, et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell, 1998, 93 : 165-176.
  • 8Romas E, Udagawa N, Zhou H, et al. The role of gpl30-mediated signals in osteoclast development: regulation of interleukin 11 production by osteoblasts and distribution of its receptor in bone marrow cultures. J Exp Med, 1996,183:2581-2591.
  • 9Liu BY, Guo J, Lanske B, et al. Conditionally immortalized mufine bone marrow stromal ceils mediate parathyroid hormone-dependent osteoclastogenesis in vitro. Endocrinology, 1998,139 : 1952-1964.
  • 10Yoshizawa T, Handa Y, Uematsu Y, et al. Mice lacking the vitamin D receptor exhibit impaired bone formation, uterine hypoplasia and growth retardation after weaning. Nat Genet, 1997,16:391-396.

二级参考文献35

  • 1Lewis DL, Hagstrom JE, Loomis AG et al . Eflqcient delivery of siRNA for inhibition of gene expression in postnatal mice [J]. Nat Genet ,2002 ;32 : 107-8.
  • 2Carmel1 MA, Zhang L, Conklin DS, et al . Germline transmission of RNAi in mice [J]. Nat Struct Biol,2003;10: 91-2.
  • 3Tiscomia G, Singer 0, Ikawa M, et al . A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA [J]. Proc Natl Acad Sci USA,2003;100: 1844-8.
  • 4Lehenkari P, Parikka V, Rautiala TJ, et al . The effects of tamoxifen and toremifene on bone ceils involve changes in plasma membrane ion conductance [J]. J Bone Miner Res,2003 ; ( 18 ) 3:473-81.
  • 5Saika M, Inoue D, Kido S, et al . 17b-Estradiol stimulates expression of osteoprotegerin by a mouse stromal cell line, ST-2, via estrogen receptor-α [J]. Endocrinology, 2001;142 (6) : 2205-2.
  • 6Lindberg MK, Erlandsson M, Alatalo SL, et al . Estrogen receptor αbut not estrogen receptor 15,is involved in the regulation of the OPG/RANKL(osteoprotegerin/receptor activator of NF-κB ligand) ratio and serum interleukin-6 inmale mice [J]. J Endocrinol,2001 ;171:425-33.
  • 7Simonet WS,Lacey DL, Dunstan CR, et al. Osteoprotegerin : a novel secreted protein involved in the regulation of bone density [J]. Cell ,1997 ;89 : 309-9.
  • 8Yasuda H, Shima N, Nakagawa N, et al . Osteoclast differentiation fac-tor is a ligand for osteoprotegerin/osteoclastogenesis inhibitory factor and is identical to TRANCE/RANKL [J]. Proc Natl Acad Sci USA , 1998;95 : 3597-3602.
  • 9Liu BY, Guo J, Lanske B, et al . Conditionally immortalized murinebone marrow stromal cells mediate parathyroid hormone dependent osteoclastogenesis in vitro [J]. Endocrinology, 1998 ; 139 : 1952-64.
  • 10Yoshizawa T, Handa Y, Uematsu Y, et al. Mice lacking the vitamin D receptor exhibit impaired bone formation, uterine hypoplasia and growth retardation after weaning [J]. Nat Gene, 1997 ; 16:391-6,.

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中华内分泌代谢杂志
2007年 第5期

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