鼻息肉中IgE及GM-CSF与嗜酸粒细胞浸润的相关性研究

Study on the correlation of IgE and GM-CSF contents with eosinophil accumulation in nasal polyp

目的:探讨鼻息肉组织中IgE和粒细胞巨噬细胞集落刺激因子(GM-CSF)的表达对嗜酸粒细胞浸润聚集的作用及二者的关系及意义。方法:采用免疫组织化学SP法检测30例鼻息肉标本(鼻息肉组)和11例下鼻甲部分切除术患者下鼻甲黏膜标本(对照组)组织中IgE阳性细胞分布情况;酶联免疫吸附法检测上述标本中GM-CSF含量,同时观测2组组织中嗜酸粒细胞的浸润程度。结果:鼻息肉组中IgE、GM-CSF水平及嗜酸粒细胞数均明显高于对照组(均P<0.01),且IgE与GM-CSF的水平分别与鼻息肉组织中嗜酸粒细胞浸润数呈正相关(r=0.65、0.62,均P<0.01),鼻息肉组中IgE与GM-CSF水平呈正相关(r=0.52,P<0.01)。结论:鼻息肉是以嗜酸粒细胞浸润为特征的疾病过程,IgE及GM-CSF则对鼻息肉中嗜酸粒细胞浸润发挥重要作用,IgE介导的Ⅰ型变态反应在鼻息肉的形成和发展中占有重要地位。

Objective：To study the effect of IgE,GM-CSF on eosinophil accumulation in the nasal polyps and find the relationship between them. Method：Thirty mucosa constitution samples of nasal polyps and eleven inferior nasal concha were observed. The distribution of IgE was detected by SP immunohistochemical method ,while the concentration of GM-CSF was measured by ELISA technique and the number of eosinophils were counted in the same tissues. Result：The level of IgE,GM-CSF and eosinophils had significant different between the nasal polyps and control group（ P〈0.01）. There was positive correlation between IgE/GM-CSF and the number of eosinophils in the nasal polyps group （ r =0.65,0.62, P 〈0.01）. The correlation between concenstration of IgE and GM-CSF was also significant in nasal polyps group（ r = 0.52, P〈0.01）. Conclusion： Nasal polyps is characterized by eosinophil accumulation. IgE and GM-CSF may contribute to the recruitment of eosinophils in nasal polyps. Type-Ⅰ allergy mediated by IgE play an important role in the pathogenesis of nasal polyps.