环氧氯丙烷对猪主动脉瓣膜去细胞基质体外酶解影响

Affection of epoxy chloropropane on degradation of acellular porcine aorta valve in vitro

目的:研究环氧氯丙烷对猪主动脉瓣膜去细胞基质体外降解的影响。方法:按实验干预不同,将90个去细胞猪瓣叶随机分为:胶原酶和弹性蛋白酶组(A组),环氧氯丙烷组(B组),水解对照组(C组)。A组将猪主动脉瓣膜去细胞基质,采用0.5%胶原酶和弹性蛋白酶降解;B组采用0.2%环氧氯丙烷预处理后48h,再应用0.5%胶原酶Ⅰ和弹性蛋白酶体外降解猪主动脉瓣膜去细胞基质;C组加入蒸馏水处理;于处理后第3、6、9、15和30天观察各组降解失重率、降解液外观、蛋白及羟脯氨酸含量。结果:A、B组的支架材料均随降解时间延长逐渐变得菲薄、组织疏松,降解液外观变浑浊,瓣膜失重率增加,降解液蛋白含量和羟脯氨酸含量增加(P<0.01);B组和A组相比在瓣膜失重率,降解液蛋白含量和羟脯氨酸含量均明显减低,但2组上述指标均高于C组(P<0.01)。结论:环氧氯丙烷有减缓胶原酶和弹性蛋白酶降解猪主动脉瓣膜去细胞基质的作用。

Objective： To investigate the affection of epoxy chloropropane on degradation of acellular porcine aortic valve in vitro. Method：According to the different treatment, 90 acellular porcine aortic valves were randomly divided into 3 groups （enzyme degradation group, epoxy chloropropane group,control group）. The acellular porcine aortic valves of enzyme degradation were degradation with 0.5% collagenase and elastase; Epoxy chloropropane group were treated with 0.2% epoxy chloropropane for 48 h before degradation with enzyme degradation; Control group were treated with water. The histomorphology, weight loss, value of protein and hydroxyproline was observed at 3, 6, 9, 15 and 30 d after degradation. Result： Epoxy chloropropane group, the acellular porcine aortic valve of enzyme group became poorer, looser and broken gradually in than control group（P〈0.01 ）. The weight loss of valve, the value of protein and hydroxyproline in vehiculum in enzyme group were significantly more than those of control group and epoxy chloropropane（P〈0.01 ）. Conclusion： The epoxy chloropropane can decelerate the degradation of collagenase and elastase in vitro.