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二氟沙星残留检测的Ci-ELISA方法建立 被引量：3

EstabLishment of Indirect Competitive Enzyme-linked Immunosorbent Assay for Difloxacin
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摘要 以合成的二氟沙星（DIF）人工抗原为基础，制备出了高效价抗DIF血清（1：2^9×10^4），并建立了DIF间接竞争酶联免疫吸附测定法（Ci—ELISA法）。标准曲线的线性回归方程为y=-0．223x-I-0．9631（r=0．9929），中值（I50）=119．21ng／ml，最低检测（LOD）=1．14ng／ml，线性范围为1．14～10000ng／ml，方法的平均批内变异系数（CV）为4．83％，平均批间CV为10．69％。DIF以浓度15～10000ng／ml在鸡肉中添加时，回收率为81．24％～90．64％，变异系数为8．18％～11．75％。 The Ci-ELISA method for the detection of difloxacin were established with the polyclonal antibodies against difloxacin. The regression equation, the 50% inhibition concentration（IC50）, the limits of detect （LOD） and the linear range about the standard curve of difloxacin were y=-0. 223x＋0. 9631（r=0. 9929）, 119.21ng/ml,1.14 ng/ml and 1.14-10000 ng/ml, respectively. The average coefficients of variation for the Ci-ELISA method were 4.83 % in intra-assay and 10.69 % in inter-assay. The recoveries in chicken ranged from 81.24 % to 90.64 %.
作者 潘孝成 祁克宗 朱良强 赵瑞宏
机构地区 安徽省农科院畜牧兽医所 安徽农业大学 安徽省兽医站
出处 《中国畜牧兽医》 CAS 2007年第10期127-129,共3页 China Animal Husbandry & Veterinary Medicine
关键词 二氟沙星 间接竞争酶联免疫吸附测定法 残留 鸡肉 difloxacin Ci-ELISA residue chicken
分类号 S859.84 [农业科学—临床兽医学]
  • 相关文献

参考文献6

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共引文献13

同被引文献32

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引证文献3

二级引证文献2

中国畜牧兽医
2007年 第10期

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