硒、维生素E对心肌内Ang-Ⅱ含量影响及其与胶原代谢关系的研究

THE RELATIONSHIP BETWEEN THE EFFECTS OF SE,VE ON ANGIOTENSIN-Ⅱ AND THE COLLAGEN METABOLISM IN INJURED MYOCARDIUM

用低硒(se)低维生素E(VE)饲料喂养大鼠60d后,实验组注射异丙肾上腺素(ISP)使心肌发生坏死。检测坏死后心肌组织中血管紧张素Ⅱ(Ang-Ⅱ)和血管紧张素I转换酶(ACE)活性,同时测定组织内胶原蛋白含量变化。结果发现低Se、VE饮食大鼠组织内Ang-Ⅱ水平升高,ACE活性也升高,组织内胶原蛋白含量增加,补Se、VE后上述改变减轻。这些结果提示,低Se、VE饮食降低了心肌细胞对损伤的耐受性,心肌坏死后Ang-Ⅱ水平升高与心肌胶原含量之间存在着相关性。

The wistar rats were maintained on low selenium (Se) and vitamin E(VE) diet from Ke-shan disease endemic area for 71 days,then they were injected isoproterenol(ISP)and killed at 3 week after injected ISP. The angiotensin Ⅱ (Ang- Ⅱ ), angiotensin-con verting enzyme (ACE) and collagen protein were determined respectively. The results showed that Ang- Ⅱ ,ACE and collagen protein content were significantly increased in injured myocardium of rats with low Se and VE diet. The results indicated that the lack of Se and VE in diet promoted myocardic injury and enhanced content of Ang- Ⅱ that induced collagen accumulate in myocardium.