微波消解-水杨基荧光酮光度法测定中草药中痕量锗

Salicyl fluorine spectrophotometric determination of trace germanium in chinese traditional herbs by microwave digestion

在H3PO4介质中,以水杨基荧光酮为显色剂,以吐温-80为表面活性剂,研究了微波消解部分清热解毒类中草药中痕量锗的测定条件。水杨基荧光酮与锗(Ⅳ)的配合物的最大吸收峰为506 nm,表观摩尔吸光系数为ε=1.54×105L.mol-1.cm-1,回归方程A=0.0409ρ+0.00614,相关系数r=0.9999,检出极限为0.014μg/mL。

In the medium of Ha PO4, the optimal experiment conditions for determination of trace germanium in some chinese traditional herbs using salicyl fluorine as colour reagent and Tween-80 as surfactant by microwave digestion has been developed. The results show that the maximum absorption wavelength of the complex of salicyl fluorine and Ge（Ⅳ） is at 506 um; the apparent molar absorptivity is 1.54× 10^5 L·mol^-1 ·cm^-1 ; the regression equation is A = 0.0409ρ ＋ 0.00614 （r = 0.9999） and the detection limit is 0.014 μg/mL.