免疫性间质性肾炎动物模型肾组织炎症细胞浸润与细胞间粘附分子-1的表达

The inflammatory cell infiltration and ICAM-1 expressing in renal tissue of immununointerstitial model

目的 探讨自身免疫性间质性肾炎的发病机理。方法 用正常小牛肾小管基底膜(bTBM)制造抗TBM型BN大鼠间质肾炎模型,并用单克隆抗体免疫组化ABC法、间接免疫荧光技术,检测炎症细胞及细胞间粘附分子-1(ICAM-1)等。结果 用bTBM免疫后第9天～3周,均可见肾间质有炎症细胞浸润及肾小管上皮细胞有ICAM-1强烈表达,与病理损害程度有同步现象。结论 在免疫性间质性肾炎发生发展中,由ICAM-1介入的细胞免疫起重要作用,但不能除外体液免疫的参与。

Objective To investigate the mechanism of immunointerstitial nephritis. Methods BN rat model was obtained with bovine tubular base membrane (bTBM). Monoclonal antibodies, immunohistochemical ABC method and indirect im-munofluorescent technique were used to detect the inflammatory cells and ICAM-1. Results 9 days after immunization with bTBM, inflammatory cells infiltration and a strong ICAM-1 expression were found in the intersitium, associated with the pathological degree. Conclusion Cellular immunity mediated by ICAM-1 may play an important role in the development of immunointerstitial nephritis. Macrophage is a leading factor among various inflammatory cells. Fluid immunity can not be excluded.