摘要
探讨急性坏死性胰腺炎(ANP)大鼠的炎性介质变化和生长抑素(stilamin)的治疗作用。方法将SD大鼠随机分为ANP组,ANP+生理盐水治疗组(ANP+NS),ANP+Stilamin治疗组(ANP+S)和正常对照组(NC)。经胰胆管逆行注射3.5%牛磺胆酸建立大鼠ANP模型。测定血的IL-1β,IL-6,IL-12,TNFα和内毒素,淀粉酶,磷脂酶A2,并对内脏标本行病理检查及电镜分析。结果在ANP组和ANP+NS组的内毒素为250±13pg/ml,230±16pg/ml;淀粉酶30000±800U/L,28000±52U/L;磷脂酶A2230±34U/L,224±28U/L及细胞因子的水平与ANP+S和NC组比较,均有统计学意义。AN+NS和ANP组可引起心、肝、肾、肺的严重损伤,而ANP+S组的多器官损伤则明显减轻。结论ANP引起多器官损伤与炎性介质过度表达有关,生长抑素对ANP的治疗有效。
Objectives To investigate the changes of inflammatory mediator in ANP and to explore the effectiveness of stilamin treatment on ANP.Methods SD rats were divided into experimental groups(ANP =12,ANP+NS=10,ANP+Stilamin=12)and control group(NC=10).Rat ANP models were made by retrograde injection of 3.5%sodium taurocholate2.5ml/kg into the pancreatic duct,and treated by intravenous injection of stilamin84μg·kg -1 ·d -1 or same amount of normal saline respectively. Serum IL 1β,IL 6,IL 12,TNF α,PLA2,amylase and endotoxin were examined.Finally,the morphology and pathology of the pancreas,liver,lung,heart and kidney,as well as the electronmicroscopical investigation of liver and lung cells were observed.Results The mean IL 1β,IL 6,IL 12, TNF α,in the ANP and ANP+NS group were higher than those in the ANP+stilamin group and Control group( P <0.01).The endotoxin,amlyase,PLA2level in the ANP+stilamin group was lower than those in the ANP and ANP+NS groups( P <0.001).ANP significantly increased white blood cell chemotax and infiltration,as well as hemorrhagic focus in liver,kidney and lung,but the pathological examinations of the liver,pancreas,lung and kidney demonstrated much less destruction in the stilamin group.Conclusions Inflammatory cytokins play an essential role to induce pathophysiological
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第5期355-358,共4页
National Medical Journal of China
关键词
胰腺炎
急性病
抑制因子.免疫
内毒素类
生长抑素
Pancreatitis Acute disease Suppressor Foctors,immunologic Endotoxins Somatostatin