摘要
To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type 3(MHV-3) induced chronic viral hepatitis in C3H/Hej mice,ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units(PFU)of MHV-3 intraperitoneally.The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin(HE) staining method from 2 days post MHV-3 infection.The ratios of T cell subsets including CD3+CD4+CD8-,CD3+CD4-CD8+,CD3+CD4-CD8-,CD3+CD4+CD25+,CD3+CD4+CD25-and CD3+ CD4-CD25+ T lymphocyte of total T lymphocytes in blood,spleen and liver were examined at 0,2,4,6,8,10,12,15,20,25,30,40 days post MHV-3 infection by flow cytosorting.We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection.The double negative T cell(DN Treg cell) and CD4+CD25+ T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice,and CD3+CD4+CD8-,CD3+CD4-CD8+,CD3+CD4+CD25-and CD3+CD4-CD25+ T cell ratios decreased accordingly.In conclusion,the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence.Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice.The increase of the DN Treg cell and CD4+CD25+ T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4+CD25+ T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection.Further characterizations of DNT cell and CD4+CD25+ T cell are under investigation.
To study the contribution of T cell subsets in the pathogenesis of Murine hepatitis virus Type 3（MHV-3） induced chronic viral hepatitis in C3H/Hej mice,ninety C3H/Hej mice were chosen to individually receive 10 plaque forming units（PFU）of MHV-3 intraperitoneally.The changes of virus titer and pathology in liver tissue were examined by standard plaque assay and by the hematoxylin/eosin（HE） staining method from 2 days post MHV-3 infection.The ratios of T cell subsets including CD3^＋CD4^＋CD8^-,CD3^＋CD4^-CD8^＋,CD3^＋CD4^-CD8^-,CD3^＋CD4^＋CD25^＋,CD3^＋CD4^＋CD25^-and CD3^＋ CD4^-CD25^＋ T lymphocyte of total T lymphocytes in blood,spleen and liver were examined at 0,2,4,6,8,10,12,15,20,25,30,40 days post MHV-3 infection by flow cytosorting.We observed that the virus titer raised and showed persistent virus duplications and inflammatory changes in the livers of C3H/Hej mice from 2 days post MHV-3 infection.The double negative T cell（DN Treg cell） and CD4^＋CD25^＋ T cell ratios increased significantly from 2 days post MHV-3 infection in C3H/Hej mice,and CD3^＋CD4^＋CD8^-,CD3^＋CD4^-CD8^＋,CD3^＋CD4^＋CD25^-and CD3^＋CD4^-CD25^＋ T cell ratios decreased accordingly.In conclusion,the changes of virus titer and pathology in the livers of C3H/Hej mice post MHV-3 suggest their contribution to viral persistence.Further characterizations of DN Treg cells are that infection indicates that MHV-3 could induce the chronic inflammation in livers of C3H/Hej mice.The increase of the DN Treg cell and CD4^＋CD25^＋ T cell ratios in C3H/Hej mice post MHV-3 infection suggests that DN Treg cells and CD4^＋CD25^＋ T cells may both have important suppressive immunomodulation functions in the development of chronic viral hepatitis and have important roles in the virus persistent infection.Further characterizations of DNT cell and CD4^＋CD25^＋ T cell are under investigation.
出处
《中国病毒学》
CSCD
2007年第5期339-346,共8页
Virologica Sinica
基金
National Nature Science Foundation (30571643, 30672380)
Major State Basic Research Development Program of China (2005CB522901,2007CB512904)
关键词
T淋巴细胞
亚群
慢性病毒性肝炎
MHV-3
MHV-3
Virus titer
C3H/Hej
Hematoxylin/eosin （HE） staining
Flow cytosorting
DNT lymphocyte
