诊断一起猪弓形虫和附红细胞体混合感染流行报告

A case report of mixed infection with toxoplasmosis and eperythrozoonsis in swine

目的对某猪场发病猪群进行诊断。方法通过临床调查、大体剖检、病理组织学观察、血清学和PCR等方法确诊。结果在血涂片中可见附红细胞体和弓形虫滋养体;在肺、肝组织切片中可见大量弓形虫假包囊;应用PCR方法检测弓形虫保守基因,结果为阳性。结论证实该猪场此次发病为猪弓形虫和附红细胞体混合感染。

In the present paper,a case of mixed infection with toxoplasmosis and eperythrozoonosis in swine is reported, in which the diagnosis of this kind of infection was made by means of clinical investigation,gross autopsy,histopathological and serological examinations as well as the PCR technique. The results showed that eperythrozoons and trophozoites of toxoplasma could be demonstrated in blood smear of the infected animals and large amount of toxoplasmic pseudocysts was visible in tissue sections of lung and liver. PCR assay used to detect the conserved toxoplasmic genes also showed positive result. All these results indicate that the infection of this pig is due to the mixed infection with toxoplasmosis and eperythrozoonsis.