纳洛酮对兔急性心肌缺血-再灌注损伤血清细胞因子的影响

Effects of Naloxone on Cytokines During Experimental Myocardial Ischemia-Reperfusion Injury

目的探讨纳洛酮对心肌缺血-再灌损伤时血清细胞因子的影响。方法利用兔心肌缺血-再灌注模型观察心肌缺血-再灌注损伤时,白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、和肿瘤坏死因子-α(TNF-α)的动态变化及用阿片受体拮抗药——纳洛酮保护和治疗时它们的变化情况。新西兰兔30只,随机分成3组(实验组、保护组和治疗组),每组10只,各组分别于缺血前、缺血后30min恢复灌流时及灌注后0.5h、1h、2h、4h、6h采血。采用放射免疫法测定IL-1β、IL-6及TNF-α含量。结果实验组冠脉结扎缺血后IL-6明显高于缺血前,而IL-1β、TNF-α则低于缺血前,再灌注后IL-1β、IL-6、TNF-α各时间段较缺血前均有不同程度升高(P<0.05);纳洛酮保护组冠脉结扎缺血后IL-1β、IL-6、TNF-α均低于缺血前(P<0.05),再灌注后IL-1β仍呈继续下降趋势,而IL-6、TNF-α则无显著性变化;治疗组注射纳洛酮后IL-1β、IL-6、TNF-α较缺血前均无显著性变化。结论纳洛酮可影响相应细胞因子的分泌而对心肌起保护作用,心肌缺血再灌注损伤前应用纳洛酮对心肌的保护作用最明显。

Objective To study the effects of naloxone on cytokines during experimental myocardial ischemia - reperfusion （I/R） injury. Methods Using myocardial ischemia models and myocardial ischemia - reperfusion injury models that were made by means of li- gating sinistral coronaria arteria, to investigate the changes of interleukin - 1β （ IL - 1β） .interleukin - 6 （ IL - 6） and tumor necrosis fac- tor - α （TNF - α） during I/R injury, and after the protection and treatment with naloxone, an antagonist of opoid receptor. 30 New Zeal- and rabbits were randomly divided into 3 groups （ischemia - reperfusion group, Naloxone protection group and Naloxone treatment group with 10 in each group）. The blood was phlebotomized at different time in each group. The concentrations of these cytokines were detected with radioimmunology method. Results The levels of IL - 6 increased after ischemia while thats of IL - 1β.TNF - α decreased, and The levels of IL - 1β, IL -6 ,TNF - α after I/R were all higher than that before ischemia（ P 〈 0.05） ; In Naloxone protection group, the levels of IL - 1β.IL -6.TNF - α were all decreased after ischemia, and the level of IL - 1β continue to decrease with different degree, but the levels of IL -6.TNF - α had no significant different with that before ischemia; The levels of IL - 1β.IL -6 and TNF - α after I/R had no significant different with that before ischemia and o. 5hr after ischemia. Conclusions Naloxone may effectively effect the levels of these cytokines after myocardial ischemia and during I/R injury ; whereby decrease the injury to vascular and myocardium. The result was most significant by inject Naloxone befor ischemia.