螺内酯对兔髂动脉球囊损伤后血管重塑及VEGF阳性表达的影响

Effects of Spironolactone on Vascular Remodeling and VEGF in Rabbits After Angioplasty in Arteria Iliaca Externa

目的揭示螺内酯对损伤的动脉血管壁血管重塑与VEGF阳性表达的影响。方法随机将20只雄性新西兰大白兔分为两组,构建兔髂动脉的球囊损伤模型。术后对照组只喂标准兔饲料,实验组喂标准兔饲料+螺内酯20mg/(kg·d)研磨稀释后经口灌胃。术后4周,活体取损伤的动脉组织。制作常规病理切片以及VEGF免疫组化切片。采用天地公司TD2000病理图像分析系统测定血管重塑的各项指标。结果实验组内弹力膜下面积、外弹力膜下面积较对照组增大(P<0.05)。实验组残余管腔面积、面积狭窄率较对照组有非常显著性差异(P<0.001)。两组中膜面积无显著性差异。实验组较对照组内皮细胞质、平滑肌细胞质内VEGF表达减弱。结论螺内酯可改善兔髂动脉球囊损伤后血管重塑。

Objective To reveal the relation between Spironolactone and Vascular Remodeling, the relation between Spironolactone and VEGF on blood vessel wall. Methods 20 male New Zealand rabbits were divided into two groups randomly. All the rabbits were contrusted to be models of angioplasty with BC. After injury, the control group were feeded with standard forage, the spironolactone group were feeded with standard forage and spironolactone[ 20mg/（kg · d） ]. The rabbits were killed one month after initial injury. The injured segments were dissected free from arteria iliaca externa, processed for histological and morphological study by imumohistochemistry and TD 2000 analysis system of pathology image. We determined several data of vascular remodeling. Results There is significant difference be- tween AUIEM, AUEEM （ P 〈 0.05 ） , extremely significant difference between residual cavity areas, ratios of stenosis （ P 〈 0. 001 ）. There is no significant difference between areas of media（P 〉 0.05）. It reduces VEGF on 28^th day. Conclusions Spironolactone can benefit vascular remodeling after angioploasty.