板蓝根化学成分的研究(Ⅰ)

Isolation and identification of chemical constituents from Radix Isatidis(Ⅰ)

目的从板蓝根中分离化学成分。方法将板蓝根的95%乙醇提取物经硅胶吸附,不同溶剂洗脱部分经硅胶柱层析分离纯化,测定所得化合物的理化常数和波谱数据,鉴定化学结构。结果从石油醚部位分得5个化合物:β-谷甾醇十二烷酸酯(Ⅰ)、十七烷酸(Ⅱ)、棕榈酸(Ⅲ)、豆甾醇(Ⅳ)、β-谷甾醇(Ⅴ)。结论化合物Ⅰ、Ⅱ、Ⅳ为首次从板蓝根药材中分离。

OBJECTIVE To isolate the chemical constituents from Radix Isatidis. METHODS The 95% ethanol extract of the plant was adsorbed by silica gel. The chemical compounds were： solated and purified by different soluents on silica gel column, and their struetures were identified on the basis of physico - chemical properties and spectral analysis. RESULTS 5 compounds were sep- arated from petroleum ether part of Radix Isatidis, and were identified as ： β - sitosteryl dodecanotate （ Ⅰ ）, heptadecanoic acid （ Ⅱ ）, palmitic acid（ Ⅲ ）, stigmasterol（ Ⅳ ）, β - sitosterol（ Ⅴ ）, respectively. CONCLUSION For the first time, Ⅰ, Ⅱ and Ⅳ were isolated from Radix Isatidis.