灯盏细辛注射液中灯盏花乙素在大鼠体内的药物动力学

Pharmacokinetic of scutellarin after iv Erigeron injection in SD rats

目的研究灯盏细辛注射液中灯盏花乙素在大鼠体内的过程。方法采用甲醇沉淀法制备血液样品,用HPLC法测定。结果灯盏花乙素50.0-0.3μg·ml^-1围内线性关系良好（r=0.9950）;样品的回收率在97%-98%,日内和日间RSD均小于5%。灯盏花乙素在大鼠体内的过程符合二室模型,主要药动学参数为：β=8×10^-4±3.1×10^-4min^-1,α=0.3695±0.0654 min^-1,T1/2α=1.875±0.856 min,T1/2β=626.12±225.64 min,AUC=2.894×103±892.1μg·ml^-1min,V（c）=21.558±2.124 ml·kg^-1,Cl（s）=0.116±0.024 m.lkg^-1·min^-1。结论灯盏细辛注射液中灯盏花乙素在SD大鼠体内过程符合静脉注射二室模型。

OBJECTIVE To study scutellarin pharmacokinetics after iv Erigeron injection in SD rats. METHODS Plasma samples by the methanol precipitation method was prepared, and detected by HPLC. RESULTS Good linearity was obtained in the range of 50. 0 -0. 3 μg·ml^-1 （r =0. 995）. The method recovery was 97% -98% ; Both the inter - day RSD and intra - day RSD were less than 5%. The concentration - time curve of scutellarin was consistent with the two - compartment model with iv Injection. The main pharmacokinetic parameters： β =8 × 10 ^-4 min^-1, α =0. 3695 min^-1 , V（c） =21. 558 ±2. 124 ml·kg^-1 , T1/2α, = 1. 875 ±0. 856 min, Cl（s） = 0. 116 ± 0. 024 kg^-1 min^- 1· ml, A UC = 2. 894 × 103 ± 892. 1 μg· ml^-1 min, T1/2β = 626. 12 ± 225.64 min. CONCLUSION Scutellarin＇ s pharmacokinetics model is two - compartment model after iv Erigeron injection in SD rats.