γ-干扰素对喉癌细胞株HEP-2增殖活性的影响

Effect of human recombinant γ-interferon on proliferative activity of human laryngeal cancer cell lines

用单克隆抗体Ki－67（抗PCNA），以链霉菌素－生物素技术（LSAB）检测喉癌细胞株HEP－2增殖细胞核抗原（PCNA）表达。人重组γ－干扰素（rhu-IFN－γ）抑制PCNA表达（即抗增殖活性）强弱与其剂量有关；雌激素对rhu－IFN－γ抗增殖作用无影响。提示：rhu-IFN－γ对喉癌细胞株HEP－2有抗增殖作用，因而在喉癌的治疗中具有应用价值的。

The effect of human on PCNA expressions of HEP-2 lines was investigated using LSAB(Labelled streptacidin biotin method ) with monoclonal antibody Ki-67(anti-PCNA). The results showed that the PCNA expression which reflects the proliferative activity of cells was dependent on dose of rhu-IFN-γ in HEP-2 cell lines. Thus,our data suggest that rhu-IFN-γ might be useful in the treatment of laryngeal cancer because lt provldes effective cytostatic.