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人类新基因CHID1的克隆与功能初步研究

Molecular Cloning and Preliminary Functional Study of Human Novel Gene CHID1
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摘要 使用生物信息学手段,从人公共蛋白质数据库的蛋白质序列中筛选到新的分泌蛋白基因CHID1(Chitinase domain containing 1),该基因定位于人11号染色体短臂15区5带(11p15.5),cDNA长1 182 bp,其编码蛋白共有393个氨基酸。转染COS-7细胞后Western blot实验显示:在细胞培养液中没有检测到CHID1蛋白。对CHID1基因在mRNA水平上进行组织分布考察发现:它在肺、肾、肝、心、胸腺中都有表达,肝中最高。细胞生长曲线揭示,Lovo-CHID1稳定细胞株有生长抑制的现象,而细胞周期分析发现细胞株在G1期发生阻滞。这表明CHID1有可能是个潜在的抑癌基因。 A set of bioinformatics methods were used to screen novel human secretory proteins from many protein sequences obtained from the public protein database in this study. A novel secretory protein gene, CHID1 was obtained. It locates in human chromosome 11p15.5, spanning 1 182 hp and encoding 393 amino acid residues. Transfecting in COS-7 cell and subsequently western blot analyzing showed that CHID1 was not detected in cell culture media. Tissue RT-PCR experiment uncovered that CHID1 was expressed in lung, kidney, liver, heart and thymus, but highest in liver. Lovo-CHID1 stable cell lines were established and cell growth curve was described. It was discovered that the growth rate of Lovo- CHID1 stable cell lines was inhibited. And cell cycle analysis supported that G1 phase of stable cell lines was arrested. So CHID1 may be a potential tumor suppressor gene.
作者 刘冬 王欣荣 万兵兵 韩泽广
机构地区 华东理工大学生物工程学院 国家人类基因组南方研究中心
出处 《华东理工大学学报（自然科学版）》 EI CAS CSCD 北大核心 2007年第5期633-638,共6页 Journal of East China University of Science and Technology
基金 国家863计划资助项目(2002BA711A0102)
关键词 CHID1 细胞周期分析 RT-PCR 基因克隆 CHID1 cell cycle analysis RT-PCR gene clone
分类号 Q78 [生物学—分子生物学]
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参考文献12

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华东理工大学学报（自然科学版）
2007年 第5期

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