阿维菌素发酵过程参数相关特性研究及过程优化

Optimization of Avermectin Fermentation Process Based on Parameter Association Analysis

微生物发酵是一个复杂的生物过程,通过对阿维菌素发酵过程中反映菌体代谢的在线参数,如OUR、CER、RQ、DO、pH以及离线参数的相关性分析,根据分析结果对发酵过程工艺进行优化,提出在发酵后期根据RQ确定碳源补加时机、根据过程pH确定碳源补加速率,使阿维菌素的发酵单位从国内平均水平3.8 g/L提高到了5.05 g/L。

Microbial fermentation is a very complicated bio-process, which was affected by the interaction of multi-scales parameters. Based on the correlation analysis of online and offline parameters, the avermectin fermentation process was optimized by adjusting carbon source feeding strategy. As a result the avermectin production was increased from 3.8 g/L to 5.05 g/L.